Broccoli Plant

ABSTRACT

The present invention provides a new broccoli variety. The broccoli plant of the present invention includes a broccoli plant identified by Accession No. FERM BP-22393 or a progeny line thereof.

TECHNICAL FIELD Related Application

This application claims priority from Japanese Patent Application No.2020-136913 filed on Aug. 14, 2020. The entire disclosure of thisJapanese patent application is incorporated herein by reference.

The present invention relates to a broccoli plant.

Background Art

Broccoli plants are cultivated in winter, harvesting from December toJanuary. However, in winter cultivation, broccoli plants tend to growslowly, and anthocyanin coloration often occurs. Broccoli plants coloredwith anthocyanin have a problem of low merchantability.

Therefore, there is a need for broccoli plants with superiorcharacteristics.

SUMMARY OF INVENTION Technical Problem

With the foregoing in mind, it is one objective of the present inventionto provide a new broccoli plant.

Solution to Problem

In order to achieve the above objective, the present invention providesa broccoli plant including a broccoli plant identified by Accession No.FERM BP-22393.

The present invention also provides a method for producing a broccoliplant, including the step of self-crossing the broccoli plant accordingto the present invention.

The present invention also provides a method for producing a broccoliplant, including the step of crossing the broccoli plant according tothe present invention with another broccoli plant.

Advantageous Effects of Invention

According to the present invention, a new broccoli plant can beprovided.

BRIEF DESCRIPTION OF DRAWINGS

FIGS. 1A and 1B are schematic diagrams showing an example of the numberof stems of a broccoli plant and an example of the length of a stem of abroccoli plant, respectively.

FIGS. 2A to 2C are schematic diagrams showing examples of the attitudeof leaves of a broccoli plant.

FIGS. 3A to 3C are schematic diagrams showing examples of the length ofbranching of a head of a broccoli plant, the shape in a longitudinalsection of the head, and the knobbling of the head, respectively.

FIG. 4 is a schematic diagram showing an example of the length ofbranching of a head of a broccoli plant, the size of the head, thelength of a peduncle of the head, and the thickness of the peduncle ofthe head.

FIG. 5 is a schematic diagram showing an example of the depth of a lobeof a leaf of a broccoli plant.

FIGS. 6A and 6B are photographs showing the deposited lines at the timeof harvest.

DESCRIPTION OF EMBODIMENTS

<Broccoli Plant>

The broccoli plant of the present invention includes a broccoli plantidentified by Accession No. FERM BP-22393 or a progeny line thereof. Thebroccoli plant of the present invention is characterized in that itincludes a broccoli plant identified by Accession No. FERM BP-22393 or aprogeny line thereof, and other configurations or conditions are notparticularly limited.

In the present invention, a “broccoli plant” is a plant classified intoBrassica oleracea var italica of Brassica oleracea of Brassica. Examplesof the broccoli plant include Brassica oleracea var. italica (Brassicaoleracea L. convar. Botrytis (L.) Alef. var. italica) and Brassicaoleracea var. cymosa Duch. (Brassica oleracea L. convar. botrytis (L.)Alef var. cymosa Duch.). The broccoli plant may be a hybrid with arelated species or a wild species, for example.

In the present invention, a “broccoli plant for cultivation,” a“broccoli variety for cultivation,” or a “broccoli for cultivation” is abroccoli plant or a variety thereof, a breeding line, or a cultivar thatis cultivated by humans and is excellent in cultivation. The “broccoliplant for cultivation,” the “broccoli variety for cultivation,” or the“broccoli for cultivation” may be a hybrid thereof, a hybrid withanother broccoli variant, or a hybrid with another Brassica oleracea.

The term “plant,” as used in the present invention, may refer to eithera plant individual representing the whole plant or a part of the plantindividual (plant part). Examples of the “plant” include plant cells,plant protoplasts, plant cell cultures or tissue cultures capable ofregenerating plants, plant calli, plant clumps, plant cells isolatedfrom plants or plant parts, leaves, pollens, embryos, cotyledons,hypocotyls, roots, root tips (tips of roots), anthers, pistils, flowers,ovaries, ovules, seeds, fruits, stems, and seedlings. The part of theplant individual may be any of organs, tissues, cells, and propagules,for example. Examples of the organs include petals, corollas, flowers,leaves, seeds, fruits, stems, and roots. The tissue is a part of theorgan, for example. Specific examples of the part of the plantindividual include microspores, flowers, flower buds, pistils, anthers,pollens, ovaries, embryos, ovules, hypocotyls, embryonic sacs, eggcells, cuttings, roots, root tips, trunks, stems, leaves, pedicles, leafmarrow, cotyledons, cells, meristematic cells, protoplasts, and seeds.The pollens may be mature pollens or immature pollens. The part of theplant individual can be, for example, derived from a plant at any growthstage, and may be derived from, for example, a pre-rooting individual, apost-rooting individual, a seedling, a cutting, or a mature individual,and the like. The part of the plant body may be one type of organ,tissue, and/or cell, or two or more types of organs, tissues, and/orcells, for example.

<Deposit Line>

The broccoli plant of the present invention may be, for example, abroccoli plant deposited under Accession No. FERM BP-22393 (depositedline) or a progeny line thereof. The information on the deposit is shownbelow. Hereinafter, the deposited line is also referred to as a broccolivariety Takii 12.

Type of deposit: International depositName of depository institution: National Institute of Technology andEvaluation, International Patent Organism Depositary; NITE-IPOD

Address: 2-5-8-120, Kazusakamatari, Kisarazu-shi, Chiba 292-0818, JapanAccession No.: FERM BP-22393

Identifying designation: Takii 12Date of acceptance: Jul. 30, 2020

The deposited lines exhibit morphological and physiologicalcharacteristics as described in Tables 1A to 1C below, for example. InTables 1A and 1B, the morphological and physiological characteristicsare based on Japanese prototypes in 2013. In Tables 1A and 1B, themorphological and physiological characteristics are evaluated based onthe Broccoli Variant Test Guideline (April 2012) published by theMinistry of Agriculture, Forestry and Fisheries of Japan (MAFF). InTable 1C, the morphological and physiological characteristics areevaluated based on the criteria to be described below. Regarding themorphological and physiological characteristics, reference can be madeto FIGS. 1A through 5.

TABLE 1A Characteristics of deposited line Characteristic No.Characteristics Deposited Line 1 Plant: number of stems One 2 Plant:height Medium 3 Leaf: attitude Semi-erect 4 Leaf: length Medium 5 Leaf:width Medium 6 Leaf: number of lobes Many 7 Leaf blade: color Gray-green8 Leaf blade: intensity of color Medium 9 Leaf blade: anthocyanincoloration Absent 10 Leaf blade: undulation of margin Medium 11 Leafblade: dentation of margin Weak 12 Leaf blade: blistering Weak 13Petiole: anthocyanin coloration Absent 14 Petiole: length Medium 15Head: length of branching Short 16 Head: size Medium 17 Head: weightHeavy 18 Head: shape in longitudinal section Transverse broad elliptic19 Head: color Green 20 Head: intensity of color Medium 21 Head:anthocyanin coloration Present 22 Head: intensity of anthocyanincoloration Weak 23 Head: knobbling Fine 24 Head: texture Medium 25 Head:firmness Firm 26 Head: conspicuousness of spiral pattern Obscure 27Head: bracts Absent 28 Peduncle: length Short 29 Peduncle: thicknessThick

TABLE 1B Characteristics of deposited line Characteristic No.Characteristics Deposited Line 30 Peduncle: hardness Medium 31 Peduncle:color Green 32 Plant: secondary heads Absent 34 Flower: color Yellow 35Flower: intensity of yellow color Medium 36 Time of harvest Late 37 Timeof beginning of flowering Medium 38 Male sterility Absent

TABLE 1C Characteristics of deposited line Characteristic No.Characteristics Deposited Line 40 Leaf: number Many 41 Stem: lengthShort 42 Leaf: depth of lobes Deep

(Characteristic No. 1)

“Plant: number of stems” refers to the number of stems immediatelybefore the time of harvest and can be evaluated by visual observation.“Plant: number of stems” can be evaluated on the basis of Note 1 (one)or Note 2 (more than one, e.g., Var.: GREEN COMET). The time of harvestrefers to the time at which half of the individuals are in the optimaltime of harvest.

(Characteristic No. 2)

“Plant: height” refers to the plant height (cm) at the time of harvest.“Plant: height” can be evaluated on the basis of Note 3 (low, e.g.,Var.: NAKAZATO WASE), Note 5 (medium, e.g., Var.: WASE MIDORI), or Note7 (high, e.g., Var.: NAKATE MIDORI).

(Characteristic No. 3)

“Leaf: attitude” refers to the angle (attitude) of the leaves at thebeginning of head formation (head appearing stage) and can be evaluatedby visual observation. “Leaf: attitude” can be evaluated on the basis ofNote 3 (semi-erect), Note 5 (horizontal, e.g., Var.: OKA MIDORI), orNote 7 (semi-pendulous).

(Characteristic No. 4)

“Leaf: length” refers to the length (cm) of the leaf, including thepetiole. “Leaf: length” can be evaluated on the basis of Note 3 (short,e.g., Var.: OKA MIDORI), Note 5 (medium, e.g., Var.: WASE MIDORI), orNote 7 (long, e.g., Var.: NAKATE MIDORI).

(Characteristic No. 5)

“Leaf: width” refers to the width (cm) of the leaf “Leaf: width” can beevaluated on the basis of Note 3 (narrow, e.g., Var.: OKA MIDORI), Note5 (medium, e.g., Var.: GREEN COMET), or Note 7 (broad, e.g., Var.:NAKATE MIDORI).

(Characteristic No. 6)

“Leaf: number of lobes” refers to the number of lobes of the leaf andcan be evaluated by visual observation. “Leaf: number of lobes” can beevaluated on the basis of Note 3 (few), Note 5 (medium, e.g., Var.: WASEMIDORI), or Note 7 (many, e.g., Var.: NAKATE MIDORI).

(Characteristic No. 7)

“Leaf blade: color” refers to the color of the leaf blade and can beevaluated by visual observation. “Leaf blade: color” can be evaluated onthe basis of Note 1 (green, e.g., Var.: GREEN COMET), Note 2(gray-green, e.g., Var.: OKA MIDORI), or Note 3 (blue-green).

(Characteristic No. 8) “Leaf blade: intensity of color” refers to theintensity of the color of the leaf blade and can be evaluated by visualobservation. “Leaf blade: intensity of color” can be evaluated on thebasis of Note 3 (light), Note 5 (medium, e.g., Var.: GREEN COMET), orNote 7 (dark, e.g., Var.: OKA MIDORI).

(Characteristic No. 9) “Leaf blade: anthocyanin coloration” refers tothe anthocyanin coloration of the leaf blade and can be evaluated byvisual observation. “Leaf blade: anthocyanin coloration” can beevaluated on the basis of Note 1 (absent) or Note 9 (present, e.g.,Var.: NAKATE MIDORI).

(Characteristic No. 10)

“Leaf blade: undulation of margin” refers to the intensity of theundulation of the margin of the leaf blade and can be evaluated byvisual observation. “Leaf blade: undulation of margin” can be evaluatedon the basis of Note 3 (weak), Note 5 (medium), or Note 7 (strong).

(Characteristic No. 11)

“Leaf blade: dentation of margin” refers to the intensity of thedentation of the margin of the leaf blade and can be evaluated by visualobservation. “Leaf blade: dentation of margin” can be evaluated on thebasis of Note 3 (weak), Note 5 (medium), or Note 7 (strong, e.g., Var.:NAKATE MIDORI).

(Characteristic No. 12)

“Leaf blade: blistering” refers to the intensity of the blistering ofthe leaf blade and can be evaluated by visual observation. “Leaf blade:blistering” can be evaluated on the basis of Note 3 (weak), Note 5(medium), or Note 7 (strong).

(Characteristic No. 13)

“Petiole: anthocyanin coloration” refers to the presence or absence ofthe anthocyanin coloration of the petiole and can be evaluated by visualobservation. “Petiole: anthocyanin coloration” can be evaluated on thebasis of Note 1 (absent) or Note 9 (present).

(Characteristic No. 14)

“Petiole: length” refers to the length (cm) of the petiole and can beevaluated by visual observation. “Petiole: length” can be evaluated onthe basis of Note 3 (short), Note 5 (medium), or Note 7 (long).

(Characteristic No. 15)

“Head: length of branching” refers to the length of branching of thehead at the base, excluding the stem. “Head: length of branching” can beevaluated on the basis of Note 3 (short), Note 5 (medium), or Note 7(long).

(Characteristic No. 16)

“Head: size” refers to the size of the head (height of head multipliedby diameter of head). “Head: size” can be evaluated on the basis of Note3 (small, e.g., Var.: GREEN COMET), Note 5 (medium), or Note 7 (long,e.g., Var.: OKA MIDORI).

(Characteristic No. 17)

“Head: weight” refers to the weight (g) of the head. “Head: weight” canbe evaluated on the basis of Note 3 (light, e.g., Var.: GREEN COMET),Note 5 (medium, e.g., Var.: WASE MIDORI), or Note 7 (heavy, e.g., Var.:OKA MIDORI).

(Characteristic No. 18)

“Head: shape in longitudinal section” refers to the shape in thelongitudinal section of the head and can be evaluated by visualobservation. “Head: shape in longitudinal section” can be evaluated onthe basis of Note 1 (circular), Note 2 (transverse broad elliptic), Note3 (transverse medium elliptic), Note 4 (transverse narrow elliptic), orNote 5 (triangular).

(Characteristic No. 19)

“Head: color” refers to the color of the head and can be evaluated byvisual observation. “Head: color” can be evaluated on the basis of Note1 (cream), Note 2 (green, e.g., Var.: NAKATE MIDORI), Note 3(gray-green), Note 4 (blue-green), or Note 5 (violet).

(Characteristic No. 20)

“Head: intensity of color” refers to the intensity of the color of thehead and can be evaluated by visual observation. “Head: intensity ofcolor” can be evaluated on the basis of Note 3 (light, e.g., Var.:GOKUWASE MIDORI), Note 5 (medium, e.g., Var.: WASE MIDORI), or Note 7(dark, e.g., Var.: OKA MIDORI).

(Characteristic No. 21)

“Head: anthocyanin coloration” refers to the presence or absence of theanthocyanin coloration of the head and can be evaluated by visualobservation. “Head: anthocyanin coloration” can be evaluated on thebasis of Note 1 (absent) or Note 9 (present).

(Characteristic No. 22)

“Head: intensity of anthocyanin coloration” refers to the intensity ofthe anthocyanin coloration of the head and can be evaluated by visualobservation. “Head: intensity of anthocyanin coloration” can beevaluated on the basis of Note 3 (weak), Note 5 (medium), or Note 7(strong).

(Characteristic No. 23)

“Head: knobbling” refers to the degree of the knobbling of the surfaceof the head and can be evaluated by visual observation. “Head:knobbling” can be evaluated on the basis of Note 3 (fine, e.g., Var.:GREEN COMET), Note 5 (medium, e.g., Var.: OKA MIDORI), or Note 7(coarse, e.g., Var.: NAKATE MIDORI).

(Characteristic No. 24)

“Head: texture” refers to the degree of texture of the surface of thehead and can be evaluated by visual observation. “Head: texture” can beevaluated on the basis of Note 3 (fine, e.g., Var.: NAKATE MIDORI), Note5 (medium, e.g., Var.: OKA MIDORI), or Note 7 (coarse, e.g., Var.:NAKAZATO WASE).

(Characteristic No. 25)

“Head: firmness” refers to the degree of firmness of the head and can beevaluated by visual observation. “Head: firmness” can be evaluated onthe basis of Note 3 (loose), Note 5 (medium), or Note 7 (firm).

(Characteristic No. 26)

“Head: conspicuousness of spiral pattern” refers to the conspicuousnessof the spiral pattern of the surface of the head and can be evaluated byvisual observation. “Head: conspicuousness of spiral pattern” can beevaluated on the basis of Note 1 (obscure) or Note 2 (obvious).

(Characteristic No. 27)

“Head: bracts” refers to the presence or absence of the bracts of thehead and can be evaluated by visual observation. “Head: bracts” can beevaluated on the basis of Note 1 (absent) or Note 9 (present).

(Characteristic No. 28)

“Peduncle: length” refers to the length of the peduncle of the head.“Peduncle: length” can be evaluated on the basis of Note 3 (short, e.g.,Var.: GREEN COMET), Note 5 (medium, e.g., Var.: JORYOKU), or Note 7(long, e.g., Var.: NAKAZATO WASE).

(Characteristic No. 29)

“Peduncle: thickness” refers to the thickness of the peduncle of thehead. “Peduncle: thickness” can be evaluated on the basis of Note 3(fine, e.g., Var.: GREEN COMET), Note 5 (medium, e.g., Var.: WASEMIDORI), or Note 7 (thick, e.g., Var.: OKA MIDORI).

(Characteristic No. 30)

“Peduncle: hardness” refers to the hardness of the peduncle of the headand can be evaluated by visual observation. “Peduncle: hardness” can beevaluated on the basis of Note 3 (soft), Note 5 (medium, e.g., Var.:GREEN COMET), or Note 7 (hard).

(Characteristic No. 31)

“Peduncle: color” refers to the color of the peduncle of the head andcan be evaluated by visual observation. “Peduncle: color” can beevaluated on the basis of Note 1 (white), Note 2 (light green, e.g.,Var.: GREEN COMET), Note 3 (green), or Note 4 (tinged with purple).

(Characteristic No. 32)

“Plant: secondary heads” refers to the presence or absence of thesecondary heads at the time of harvest and can be evaluated by visualobservation. “Plant: secondary heads” can be evaluated on the basis ofNote 1 (absent) or Note 9 (present, e.g., Var.: NAKATE MIDORI).

(Characteristic No. 34)

“Flower: color” refers to the color of the flower at the time offlowering and can be evaluated by visual observation. “Flower: color”can be evaluated on the basis of Note 1 (white) or Note 2 (yellow).

(Characteristic No. 35)

“Flower: intensity of yellow color” refers to the intensity of theyellow color of the flower and can be evaluated by visual observation.“Flower: intensity of yellow color” can be evaluated on the basis ofNote 3 (light), Note 5 (medium), or Note 7 (dark).

(Characteristic No. 36)

“Time of harvest” refers to the time of harvest (the time at which halfof the individuals are in the optimal time of harvest). “Time ofharvest” can be evaluated on the basis of Note 3 (early, e.g., Var.:WASE MIDORI), Note 5 (medium, e.g., Var.: OKA MIDORI), or Note 7 (late,e.g., Var.: NAKATE MIDORI).

(Characteristic No. 37)

“Time of beginning of flowering” refers to the time of beginning offlowering (the time at which 50% of the individuals are with at least10% of small flowers). “Time of beginning of flowering” can be evaluatedon the basis of Note 3 (early, e.g., Var.: GREEN COMET), Note 5 (medium,e.g., Var.: NAKATE MIDORI), or Note 7 (late).

(Characteristic No. 38)

“Male sterility” refers to the presence or absence of male sterility andcan be evaluated by visual observation. “Male sterility” can beevaluated on the basis of Note 1 (absent) or Note 9 (present).

(Characteristic No. 40)

“Leaf: number” refers to the total number of leaves. “Leaf: number” canbe evaluated on the basis of Note 3 (few, about 16, e.g., Var.: OHAYO),Note 5 (medium, about 22, e.g., Var.: GRANDOME), or Note 7 (many, about26, e.g., Var.: OKUMIDORI 100).

(Characteristic No. 41)

“Stem: length” refers to the length of the stem from the ground edge tothe base of the small flower head at the lowermost part of the mainhead. “Stem: length” can be evaluated on the basis of the Note 1 (short,about 16 cm, e.g., Var.: OHAYO) or Note 9 (long, about 26 cm, e.g.,Var.: PIXEL).

(Characteristic No. 42)

“Leaf: depth of lobes” refers to the distance from the center point ofthe line connecting the apexes of the leaf blades above and below thelobe of the leaf to the position closest to the main vein of the lobe,and also refers to the deepest lobe when there are two or more lobes.“Leaf: depth of lobes” can be evaluated on the basis of Note 1 (shallow,about 2 cm, e.g., Var.: OHAYO) or Note 9 (deep, about 7 cm, e.g., Var.:CHALLENGER).

The deposited line has self-incompatibility. The S genes forself-incompatibility of the deposited lines are an SRK¹⁸ gene and anSLG¹⁸ gene. Regarding the SRK¹⁸ gene and the SLG¹⁸ gene, reference canbe made to Reference 1 below. The base sequences of the SRK¹⁸ gene(Genbank Accession No.: AB032473.1) and the base sequences of the SLG¹⁸gene (Genbank Accession No.: AB032471.1) are, for example, the basesequences of SEQ ID NO: 1 and SEQ ID NO: 2, respectively.

-   Reference 1: Tohru Suzuki et al., Characterization of Brassica    S-haplotypes lacking S-locus glycoprotein, FEBS Letters, 2000, vol.    482, pp. 102-08.

SRK¹⁸ gene (SEQ ID NO: 1)5′-ATGAAAGGTGTACGAAACATCTATCACCATTCTTACACCTCCTTGTTGCTCGTCTACGTTGTCATGATTCTATTTCATCCTGGCCTTGCGATCTATATCACCACTTTGTCGGCTACAGAATCTCTTACAATCTCAAGCAACAGAACACTTGTATCTCCCGGCAATGTCTTCGAGCTCGGTTTCTTCAAAACCACCTCAAGTTCCCGTTGGTATCTCGGGATATGGTACAAGAAATTGCCCGACAGAACCTATGTATGGATTGCCAACAGAGATAACCCTCTCCCCAATACCATTGGAACCCTCAAAATCTCAGGCAATAATCTTGTCATCCTTGGTCATTCCAATAAATCTGTTTGGTCGACGAATGTAACTAGAGGAAATGAGAGATCTCCGGTGGTGGCAGAGCTTCTCGCTAACGGAAACTTCGTGATGCGAGACTCCAATAACACAGACGCAAATGAATTCTTGTGGCAAAGTTTCGATTTCCCTACAAATACTTTGCTTCCAGAGATGAAACTGGGTTACGACCTCAAAACAGGGCTGAACAGGTTCCTTACATCATGGAGAGGTTCAGATGATCCGTCAAGCGGGGATCACTTGTACAAGCTCGAACCCCGAAGTTTTCCTGAATTTTATATATTTAACGACGACTTTCCAGTGCATCGGATTGGTCCATGGAATGGAATCGAATTTAGTGGCATACCAGAGGACCAGAAGTCGAGTTACATGGTGTACAATTTCACAGAGAATAGTGAGGAGGTTGCTTATTCATTTCGAATGACCAACAACAGCATTTACTCGAGATTGATAATAAGTTCCGAAGGGTATTTACAGCGACTGATATGGACTCCGTCAACAAAGATATGGCAAGAGTTCTGGTCTTCTCCAGTGAGCCTCCAGTGCGATCCATACAGGATTTGTGGGCCTTACGCTTACTGTGACGAGAACACATCACCGGTGTGTAACTGTATACAAGGGTTCGATCCCAAGAACCAGCAGCAGTGGGATCTGAGATCCCATGCAAGTGGGTGTATAAGGAGGACGTGGCTGAGCTGCCGTGGTGATGGTTTTACAAGGATGAAGAATATGAAGTTGCCAGACACTACGGCGGCGATTGTCGACCGGAGTGTTGGTGTGAAAGAATGTGAGAAGAAATGCCTTAGCAATTGTAATTGTACTGCATTTGCAAATGCGGATATCCGGAATGGTGGGACGGGTTGTGTGATTTGGACCGGGGAGCTTGAAGATATCCGGAATTACGTTGCTGACGGTCAAGATCTTTATGTCAGATTAGCTGCTGCTGATCTCGTTAAGAAGAGAAACTCGAATGGGAAAATCATAGGTTTGATTGTTGGAGTTAGTGTTCTGCTTCTTCTAATAATTTCCTGCCTCTGGAAAAGGAGACAAAAGCGAGCAAAAGCAAGTGCAACATCTATTGCAAATCGACAGAGAAACCAAAATATGCCTATGAACGGGATGGTGCTATCAAGCAAGAGACAGTTGTCTGGAGAGAACAAAATTGAGGATTTGGAACTTCCATTGATAGAGTTGGAAGCTGTTGTCAAAGCCACCGAAAATTTCTCCAGTTGTAATAAAATCGGAGAAGGTGGTTTTGGTATTGTTTACAAGGGAAGATTACTTGATGGGCAAGAAATCGCGGTAAAAAGGCTATCAAAGACGTCATTTCAAGGGACTGATGAGTTTATGAATGAGGTGACATTAATCGCAAGGCTTCAGCATATAAACCTTGTTCAAGTTCTTGGCTGTTGCATTGAAGGAGATGAGAAAATGCTGATATATGAGTATTTGGAAAATTTAAGCCTCGATTCTTATCTCTTCGGAAAAACCCGAAGCTCTAAGCTAAGTTGGAAGGAGAGATTCGACATTACCAATGGTGTTGCTCGAGGGCTTTTATATCTACATCAAGACTCACGATTTAGGATAATCCACAGAGATTTGAAAGTAAGTAACATTTTGCTTGATAAAAATATGATCCCAAAGATCTCGGATTTTGGGATGGCCAGAATATTTGCAAGGGATGAGACGGAAGCAAACACAATGAAGGTGGTCGGAACTTACGGCTACATGTCCCCAGAGTATGCAATGAATGGGATCTTCTCAGAAAAATCAGATGTTTTCAGTTTTGGAGTCATAGTTCTTGAAATTGTTACTGGAAAGAGGAACAGAGGATTCTACAACTTGAACTACAAAAACAATTTTCTAAGCTATGCATGGAGTAATTGGAAGGAAGGAAGAGCGCTAGAAATCGTAGATCCAGTCATTGTAGATTCATTGTCACCACTGTCATCAACATTTCAACCACAAGAAGTCCTAAAATGCATACAAATTGGTCTCTTGTGTGTTCAAGAACTTGCAGAGCACAGACCAACGATGTCGACTGTGGTTTGGATGCTTGGAAGTGAAGCAACAGAGATTCCTCAGCCTAAACCGCCAGGTTATTGCGTCGGAAGCAGTCCTTATGAACTAGATCCATCAGCAAGTAGGCAGTTGGACGATGATGAATCCTGGACGGTGAACCAGTACACTTGCTCAGTCATCGATGCCCGGTAATATGAACGCTGTTGAGGAAGTTCATATAATTAAACATTACTAAATGCAGTGACTCAATATCATATGTGAAAGAAGGAAATAAATTCTCAAAATATAAGTATGTTATTTTGTAAC-3′ SLG¹⁸ gene (SEQ ID NO: 2)5′-ATGAAAGGCGTGAGAAAAACCTACGATAATTCTTACACCGTAACCTTTTTGCTTGTCTTTTTCGTCTTGATCCTATTTCGTCCTGCCTTTTCGATCAACACGTTGTCGGCTACAGAATCTCTTACAATCTCAAGCAACAGAACACTTGTATCTCCCGGCAACGTCTTCGAGCTCGGCTTCTTCCGAACCACCTCAAGTTCTCGTTGGTATCTCGGGATATGGTACAAGAAATTGCCCGACAGAACCTATGTATGGGTTGCCAACAGAGATAACCCTCTCTCCAGTTCCACTGGAACCCTCAAAATTTCAGGCAATAATCTTGTCATCCTTGGCCACTCCAATAAATCTGTTTGGTCGACGAATGTAACTAGAGGAAATGAGAGATCTCCGGTGGTTGCAGAGCTTCTCGCTAATGGAAACTATGTGATACGAGACTCCAATAACAAGGACGCAAGTGGATTCTTGTGGCAAAGTTTCGATTTCCCTACAAATACTTTGCTTCCAGAGATGAAACTGGGTTACGACCTCAAAACAGGGCTGAACAGGTTCCTTACATCATGGAGAGGTTCAGATGATCCGTCAAGCGGGGAAATCACTTACAAGCTCGAACCCCGAAGGTTTCCTGAGTTTTATATATTTAGCGACGACTTTTGAGTGCACCGGATTGGTCCATGGAATGGAATCGGATTTAGTGGCATACCAGAGGACCAGAAGTCGAGTTACATGGTGTACAATTTCACAGAGAATAGTGAGGAGGTTGCTTATTCATTTCAAATGACCAACAACAGCATTTACTCGAGATTGATAATAACTTCCGAAGGGTATTTACAGCTACTGATGTGGACTCCGTCAACAAAGATGTGGCAAGAGTTCTGGTCTTCTCCAGTGAGCCTCCAGTGCGATCCATACAGGATTTGTGGGCCTTGCGCTTACTGTGACGAGAACACATCACCGGTATGTAACTGTATACAAGGGTTCTATCCCAAGAACCGGCAGCAGTGGGATGTGAGAGTCGCTTCAAGTGGGTGTATAAGGAGAACACGGCTGAGCTGCAGTGGAGATGGTTTTACCAGGATGAAGAACATGAATTTGCCAGACACTACAATGGCGACTGTAGACAGGAGCATTGATGTAAAAGAATGTAAGAAGAGATGCCTTAGCGATTGTAATTGTACCGCTTATGCAAATGCGGATATCCGGAATGGTGGGACGGGTTGTGTGATCTGGACCGGAGCGCTTGAGGACATCCGGACTTACTTTGCTGAAGGTCAAGATCTTTATGTCAAATCGGCTGCTG CTGACCTTGCTTAG-3′.

In the present invention, plants having “essentially all physiologicaland morphological characteristics of the deposited line” are meant to beplants having the main characteristics of the deposited line when grownin the same environment. The main characteristics are the followingcharacteristics of (1) to (10), i.e., characteristics of CharacteristicNos. 6, 9, 13, 21, 25, 28, 36, and 40 to 42, and self-incompatibility.The main characteristics are preferably characteristics ofCharacteristic Nos. 9, 13, 21, and 36 in Tables 1A and 1B, i.e., thefollowing characteristics of (1) to (3) and (9) andself-incompatibility. The plants having essentially all physiologicaland morphological characteristics of the deposited line may be, forexample, plants having the same characteristic as the deposited line,except for 10 or less, 9 or less, 8 or less, 7 or less, 6 or less, 5 orless, 4 or less, 3 or less, 2 or less, or 1 characteristic, i.e., 10 orless, 9 or less, 8 or less, 7 or less, 6 or less, 5 or less, 4 or less,3 or less, 2 or less, or 1 characteristic may differ from the depositedline. The “characteristic different from the deposited line” may be amain characteristic of the deposited line or a characteristic other thanthe main characteristic of the deposited line, and is preferably acharacteristic other than the main characteristic of the deposited line.The “characteristic different from the deposited line” can be made, forexample, by the introduction of a characteristic and/or introduction ofa gene, which will be described below. In the plants having essentiallyall the physiological and morphological characteristics of the depositedline, all the characteristics of Characteristic Nos. 1 to 21, 23 to 32,34 to 38, and 40 to 43 and self-incompatibility may be the same as thedeposited line.

(1) anthocyanin coloration of leaf blade: absent;(2) anthocyanin coloration of petiole: absent;(3) intensity of anthocyanin coloration of head: weak;(4) number of leaves: many;(5) length of stem: short;(6) firmness of head: firm;(7) number of lobes: many;(8) depth of lobe: deep;(9) time of harvest: late; and(10) self-incompatibility gene: SRK¹⁸ gene and SLG¹⁸ gene.

The deposited lines have, for example, the single nucleotidepolymorphisms (SNPs) described in Tables 2A through 2U below. In Tables2A through 2U, the base [N₁/N₂] in parentheses denotes a single basepolymorphism in which N₁ is a base in a dataset (Brassica oleracea L.:genome base sequence of TO1000) registered in NCBI with RefSeq assemblyaccession: GCF_000695525.1 (https://www.ncbi.nlm.nih.gov/assembly/GCF_000695525.1/) and N₂ is a base otherthan the base in the dataset. For the analysis of the data set,reference can be made to Reference 2 below, for example.

-   Reference 2: Isobel A. P. Parkin et al., Transcriptome and methylome    profiling reveals relics of genome dominance in the mesopolyploid    Brassica oleracea, Genome Biology, 2014, vol. 15, no. 6, art. no.    R77.

TALBE 2A Location of SNP on SEQ Deposited SNP ID Chr cM chromosomeID NO: [SNP] and its adjacent sequences (5′ → 3′) A B line SNP1 1  0.990  454335 SEQ ID TGACTTGTGTCGGTGGCTAGAGGAAAAGGCCCCTTTGTCCAC G T A NO: 3AAACGTGT[T/G]GTCTGTCTGGGCCATTCATTTCAAGCCCA TGTATTACGGTTTTAATTACG SNP2 1 6.422  1264234 SEQ ID TTTGAAACAACAAGCAGATAACCCAAAGCAACTTCTTGCTGA G C BNO: 4 GCTAATGG[G/C]CATGGGCATCAGCTAATAAGGCAAGTGCT AGTGACAAGGCTGTTGCTACTGSNP3 1  9.780  1783522 SEQ ID ATTCTCTGTTCTTGATCATGCGCTTGTGTCTTCTATTCCCTGC T B NO: 5 AACCTTCA[T/C]TGGACTCTTGACCTCTTTTCTTACCAAAACAAGTAAAATCCTCAAGAGAA SNP4 1 24.743  4314082 SEQ IDACGATGTGCTGGAGGATGGAAGTGAAGTGGAAAATTTGTTGA T G A NO: 6AAAATGCC[T/G]CAGAGAAGACAGCAGAGGCGCTGCAGGCG AAAGACGAGTATGAGAAACAT SNP5 136.521  7889954 SEQ ID TCTTGCAGCCTAATCTTTCGGCTATCCCATGGCACTCGGTGA A C ANO: 7 TTTGGCTC[A/C]AAGAGAGGATACAAAAGCACGCTTTTATT TCATGGGTCCTTGTGAGACACSNP6 1 39.178  8784702 SEQ ID TGAGATTTGGAGCTGCAGTGATCACAGACTCTAGCGACCGTCT C B NO: 8 GGACAGCG[C/T]TTAGTAGAGTGACCTATTTCTTTGCATAACTCACATACCGGTGGCATCCA SNP7 1 40.958  9420949 SEQ IDAAGTTTGGATCGGACGCAATCGCTGCACTGGCGGCACTAACG C T A NO: 9CTCTCGGC[C/T]ATCGAAGAAGTTGCAGCCATCTGCAGCGG CTGAGCGGGTAACTGTAAACC SNP8 146.689 11980318 SEQ ID AACCGGAACATTCCGTAGTCTGCAGCGTACCAGAACTTAGCT A G ANO: 10 CGGTTCTC[G/A]GCAGGAAAGTAGAACATGGTCTCTGCGGG AACACCACTCTCCCCCAGASNP9 1 52.193 13607081 SEQ ID TCAGCCTGGCGTCTCTCTCCAGCTCCAAGCTGAGCCCAGTTCG C B NO: 11 AGCCTGAA[G/C]GTCTGCTCACACCCCGGCATTTGAGCACCATTGTAGGTCTGCAAAATCCT SNP10 1 52.272 13635739 SEQ IDTGAGACCAAGGTCATAGAGCGAGCCGAACTCTGAGTGGACAG C G B NO: 12AAGAAACC[C/G]ACATCAACATCAGCCAAGCGTAACTAATA GTTACAAGGTGGATCTCTGCA

TABLE 2B Location of SNP on SEQ Deposited SNP ID Chr cM chromosomeID NO: [SNP] and its adjacent sequences (5′ → 3′) A B line SNP11 156.649 17705704 SEQ ID CGTTTGGGAAATGCGCGATGACGTGATCTCGTTTGCGTTTCTT A G BNO: 13 CTGCTTC[A/G]TATCTCACTCGCATCTCGAGCTCGGGCCTTC GTCTCTGCAGCTGCGGGACSNP12 1 58.660 22288471 SEQ IDTCGTTAAATGCTGCAGTTTCCATGCCAAGCTTCTGATCTTCAA G T A NO: 14ATGGTGT[G/T]TCGTCAACTTTTTCTAATCGCTGAGGAAGAG GCGTTGGCATAGGATTGCA SNP13 175.912 41231290 SEQ ID TCTCCGGCAACAGCCGATGGACCTGTTTGCCCACCGACGACAA T C BNO: 15 AACTAAG[T/C]CGGGCAAGTTTCCCTGAAGGTTTCCTATTTG GCACGGCTACTGCAGCATASNP14 1 77.423 41526625 SEQ IDTGTCTGAGAGGCAAAAATGCCAAGATCAGAGTGGTGAAAATGA C A A NO: 16ACTATTC[A/C]TAGCACATAACAAATGAAGTAGTCTTCTATG ACCATCACACTATACTACA SNP15 182.329 42118296 SEQ ID CTGCAGATACAGACGGAGGATTGCGTTAGATCGGGATCGGGAG G T ANO: 17 GGAGCAA[G/T]GAGATCGATCGGTTGCCGTGGAAAGGAGGGA GCGAGGGGAATCCTGATTASNP16 1 87.796 42587881 SEQ IDTATGTGGGAGTCATTGCTTGAACCGCTTCTGAGTCCTTTGTTG C G B NO: 18ACACACT[G/C]TCAGCGAGCTCTTGACTCCTCGTGGACTAGT CTTCTACGGGAAGGCAGGG SNP17 188.361 42631525 SEQ ID AGTACGACCGCACCTGGTTTGAAAATTTCCATCACTTTCCCCA C T ANO: 19 TGATGGG[T/C]TTGAACAAGAGATGATAGCTCTCGTCGTCGA TACCATCGTCCAGTGGTACSNP18 1 88.361 42631552 SEQ IDTCCATCACTTTCCCCATGATGGGTTTGAACAAGAGATGATAGC A G A NO: 20TCTCGTC[G/A]TCGATACCATCGTCCAGTGGTACGTTGAGAG AATAGTACTTTCCACTTCC SNP19 195.612 43132615 SEQ ID AGTCCTCTGCAGCATAATCAGAGTAAGCAAAGAAGAGCAGGCT T A BNO: 21 TGGAGTG[A/T]CGAAAGAGATGCTCTTTGGGTTCTGAATGGT TTTCATGCCCGTGGTGTGASNP20 2  3.410   717479 SEQ IDATAACTTCCACAATCGCTGGTGTTGCCGTCTCTAACGCTAACC T A A NO: 22TATTCGG[T/A]ATTGCAAACGGAACAGCACGAGGCGGTGTTC CCTCTGCTCGAATAGCCAC

TABLE 2C Location of SNP on SEQ Deposited SNP ID Chr cM chromosomeID NO: [SNP] and its adjacent sequences (5′ → 3′) A B line SNP21 2 3.410   717482 SEQ ID ACTTCCACAATCGCTGGTGTTGCCGTCTCTAACGCTAACCTAT T A ANO: 23 TCGGTAT[T/A]GCAAACGGAACAGCACGAGGCGGTGTTCCCT CTGCTCGAATAGCCACTTASNP22 2  8.829  1481256 SEQ IDGAAGCGAGTGCGATGAGTGCAAGACCTCCTACGATTGCTGCCG A T B NO: 24TGTTCTC[A/T]GCCCCATCGGACGAAGATCCTGCAGTGCCTG AGCTTATGGCGCTCTCTGC SNP23 232.758  3792354 SEQ ID AGTGAAGAACCCTGCAGAGAAGACTCGAGTAGTTTCTCCGTGT G A BNO: 25 TTGCTTG[G/A]TTTGAGGCGTTTGAGAGAAGAAGAAGTGGCG GAAGCAGCTATAGAACAGTSNP24 2 49.203  8267341 SEQ IDGCTGAAAAACGAACTAGCCGCGATCATTGTGAAGAGGGCATCA A G A NO: 26CTAAATC[G/A]CAATGCAGTAAATAAAGAGGTTGAAGAAGAT AACATCAAAGACATTAGTG SNP25 249.415  8420520 SEQ ID TTGCTGCAGCTTTGTGAGGAGAGTGTGGTGTATCGAACCATGG A C BNO: 27 TGGCGCG[A/C]GAAGGTGCGATTGCTCCTGTGGTGGCTTTGT CGCAGAGTAGTAAGAGTCGSNP26 2 49.704  8638214 SEQ IDGGAAGCACGAGTTTTGCGGCTGTGGAGGACAGCATAGCACACG T C A NO: 28GTGTTGGG[T/C]GATGCCTAGCTGGGATCGCCTCTTGTAGAG CGTAGGAGAGGCAATGAGTT SNP27 251.821 11081737 SEQ ID ATGACCTACGACAAGTAGACAAGATGTGTGTGTCCGAAACAGG G A ANO: 29 CGGCTCA[A/G]GCAAGTTAGTTTTCAGGAATGCATCAACCAC AACAGGTGAGGCATATGTTSNP28 2 55.999 27333228 SEQ IDCACCATAAAAGCTGCAGCAGCCTCTTCAACAACATTTTTCTTC G A A NO: 30TTCCTT[G/A]CACGATCCTCTCCTAGAGGGTTCTCCTACTTC TCCTCTTCTTTTCAAAGA SNP29 262.589 45424768 SEQ ID CTCATTGCTCGTACACTGCAAATGTTCATGTCTCTGCTCCTGC A G ANO: 31 TGTGTCC[G/A]GCAGATTTGTACTTCTATACGGTCCTCAGAT AGTTGGCTTCTCATTTGCCSNP30 2 64.493 46653493 SEQ IDGAGCAAGAGACTGTGATGAGGTTCCTGTGCTGTAAATGTTGTA A T B NO: 32GAAAGA[A/T]TCTGATGGTCCACAAACTGAGGAGAAGAGTAG TTCTGTATCTGAGGCTGC

TABLE 2D Location of SNP on SEQ Deposited SNP ID Chr cM chromosomeID NO: [SNP] and its adjacent sequences (5′ → 3′) A B line SNP31 274.571 49757428 SEQ ID TCACGGTGGCTGAAAAAACTTCGGGATCAGCATAAAAGATCCA A G ANO: 33 CCGGAGG[G/A]GAGGTGTTCGGAGGAGGGAAACATGCGACGG GATGGTGGCTTCCGGGGAASNP32 2 77.901 50407246 SEQ IDAGGTCAGAATCAGTGACACCTGGATAAAACGACCTGGAGACTG G A B NO: 34ACTGAGA[G/A]AAGTCCAGCTCGACGATTTGAGTGAACCTGA GGGCGAGACGGCCAAGCAA SNP33 283.240 51346970 SEQ ID GTTCCTATCCTTTGCAAGAGCGTGATCCCGGTGTCTATACCGG C T ANO: 35 TGATTCT[C/T]CTCAAGGTAGGCACGAGGACGCGGTCGTAGA TTATGATGAAAACAGTCATSNP34 2 83.240 51347002 SEQ IDGTCTATACCGGTGATTCTCCTCAAGGTAGGCACGAGGACGCGG T G B NO: 36TCGTAGA[T/G]TATGATGAAAACAGTCATTCCCGTCATCAAG AAGACCACATAGGTGGCTG SNP35 284.263 51516676 SEQ ID AGTCCAACGCGGTCGTAGTCAACGGGATCGTTGACCGCTCGTT A G BNO: 37 GGTGAGA[G/A]ACAGCCGTTGACGCGATTACGTTGCGGTGAG TGAGGAGAACGCCTTTGACSNP36 2 84.263 51516714 SEQ IDTCGTTGGTGAGAGACAGCCGTTGACGCGATTACGTTGCGGTGA T G B NO: 38GTGAGGA[G/T]AACGCCTTTGACTTTCCCCGTGGTCCCGGAC GAGAAGAGAATCGCTGCAG SNP37 286.232 51834225 SEQ ID CTTGGTATTGTGCAGTTTATCCCAACACGTTTGACTCTGGATT A G ANO: 39 CAGCGGT[A/G]ACAAGGCCACCACCGCTATTGTGTGAAGCTG ACTTACTGAGTTGCATGGASNP38 2 86.232 51834270 SEQ IDGCGGTAACAAGGCCACCACCGCTATTGTGTGAAGCTGACTTAC C T A NO: 40TGAGTTG[C/T]ATGGACAAGGTGAGTAAAACTGTCTTGTTTG CCCCTCTTTTCATGGTGGT SNP39 3 1.187   675632 SEQ ID GCCTCCTCGTTCCAGCGTTGTAACGAGACACAAAACGCTTTAG A C BNO: 41 TGAGACT[A/C]CACAACAGCTTGTTGCAACAAGCCGTGATAA AGCTCAATAACGAAGCCAASNP40 3  2.498   948354 SEQ IDGTCGTCTCTGCCCTCCCCGGCGGGTTCAACGAGATCGATCCCG T C B NO: 42TCGCCGT[C/T]GTCGTGGTCCTCGCGATCACCGTCATCATCT GCTGCAGCACGAGGGAGAG

TABLE 2E Location of SNP on SEQ Deposited SNP ID Chr cM chromosomeID NO: [SNP] and its adjacent sequences (5′ → 3′) A B line SNP41 3 5.739  1516935 SEQ ID GTGGTTGAAGGACAGCCAACGCAGCCAGAGCAAGGTAACCAAG G A BNO: 43 CCACGTC[A/G]CTACTCGTGTCCATGGCCAGGAGTAGAGTCT GAGCCGGAGTTCCCGATCTTSNP42 3  5.739  1516977 SEQ IDGCCACGTCACTACTCGTGTCCATGGCCAGGAGTAGAGTCTGAG A T B NO: 44CCGGAGT[T/A]CCGATCTTAGCCTTGACAATGTACGTTGCGC TCTGCAGCATTTGACGCCC SNP43 320.656  3369359 SEQ ID CGGGTGTGGGAGCCGGAGGAGAGGCAGCAGAGTCGGTGGGTGC A G ANO: 45 CTCGCCA[G/A]AATCACTGGGACTAGCCGCGGAGTAGTCATC CTCAGCAGCGGGTCCTTCTSNP44 3 21.013  3409356 SEQ IDTCTGTTTAGAGGCAGTGGACTTGAGTTTCCGGTCTGGCTTCAC T G B NO: 46CGTCTCA[G/T]CGCGTTTAGGTTTAGCGTTCTTGTCCACAGG AGAAGACTTCTCTGCCTCA SNP45 328.303  4315752 SEQ ID TTGAACTTGCCGTGTTTGAACTTACCGTGACCATGATACCCGT T G ANO: 47 AGCCATA[T/G]CCGTGACCATGGTGGTGACCATAGTGTCCGT GGTGAGACATATGGTGAGASNP46 3 38.853  5932635 SEQ IDGTGACCAAGCTAACAAAGCGAGTCCAGGAATGTTCCAATTCTG G A A NO: 48TGTCCAC[G/A]CAAGAGCTGAATAAAGGGCATGAGCTTATTC ACCAAACGCCAAAACAAAT SNP47 348.248  8135062 SEQ ID GCTGGTCGGAACTCATGGAGAGTGAGTAAATTTTCTTCTTTAC T G BNO: 49 ACGAGAA[T/G]GAATCCATCCATGGCTCAAATCTTGATCGGT TTCAGGGTACGTTGAAGAASNP48 3 49.393  8510724 SEQ IDAGTCACCGGAGAGGACCCGGGTTCAAGGGAGCCACTCTAGTAA C T A NO: 50TAGTAG[T/C]AATACCTGGGGGAGGTTCGGCGGGAACAGCAG GGCGGTTGTGACGAAGAC SNP49 354.259 10912440 SEQ ID AGTTGGATCCTCAACGTTTGCCTTCTTTGGGTTCAACGGTAAT T G BNO: 51 GACATTC[T/G]CAATCTCATTACTTTTCTGAATAAAGCTTTT TTCTTATTGTGTGAAACTASNP50 3 56.441 12711867 SEQ IDCTGCAGATTATTGTTCAATACACTATACTATTGGGAGGTGGCT C A B NO: 52GTAAGGT[A/C]TTATGGGCATTGAAGGTGGGAACACAAGTTC AAAATCTGGAAATGTGGAC

TABLE 2F Location of SNP on SEQ Deposited SNP ID Chr cM chromosomeID NO: [SNP] and its adjacent sequences (5′ → 3′) A B line SNP51 359.622 14684781 SEQ ID TCTCCTGCATCGAAGGTCCAGTGAACCGCATAGTGTGTACGTT A T BNO: 53 CATTTGT[T/A]TGAAACGCCAGAGAGGAAATGTAACCATTGT GTATCTCCCCGCATATCCCSNP52 3 68.614 20818643 SEQ IDGCGGCACAAACGGAGCAGGATCTTACTTCTGAGAAGAGAGTAT T C B NO: 54GCGTTCG[C/T]GATCGATTCACCTTTTGCTAGGGATCGATTT CCTGGCTTAGTATACTTTG SNP53 371.069 22019198 SEQ ID AGCCAATGAGCTTGTGGACTTCATGGAAGCCTCTGGGGATCTT A G BNO: 55 CTGGATG[A/G]CAAAGCAATGGCGTCTTTAGTCGAAGGGCAT TGCGATGCCAAAGATCTCGSNP54 3 75.613 23508195 SEQ IDTGTTGGAGAGTGCAGTTTATGACTCTAATGCCGCTGACATCCT T G A NO: 56GTTCGTT[T/G]CCGTACCTTCCAAGGCTTCCGACACTGATTC CATGTCCTGGACCGCAGGT SNP55 376.384 23746683 SEQ ID AGAGTCTTCCTTGGGGTTGGAGTTGGTGACGACAGGTCCGCTA C T BNO: 57 CAGTACT[C/T]CCTGCCGGACTCACCGGATCCGTCGCCCAAC CCTTCTCCATATCTGGTTCSNP56 3 76.582 23809957 SEQ IDTTCTTCTCAGTGGCACTACCATTTGCTGCAGAAGCTCTGAGAA T C A NO: 58GAGGCTC[C/T]AAGAGCTTGCTTGCCACATCAGGAGGCAAAG CGTCTTCACCGGGAAGAAC SNP57 379.755 24764254 SEQ ID CTAGCATCAACCTCTGTCCCGCCAGTCCCTGTCACAGCTGCTC T G ANO: 59 TATCCCC[T/G]GCCTGCCCTATCTCATCTGCCCTAGCCCCTG CTGCAGGTTCTTCGGGCTGSNP58 3 80.244 24921510 SEQ IDACACATCCACAATCTCCTCAAACGAACAACGCCTCATGCTACG T C A NO: 60GCCTCTA[T/C]AATCTTGAGCATAGTTCCTCAAGTCCAAAAG CTCTCCGATCTGCAGCTCA SNP59 383.407 26569816 SEQ ID CTCCGCCGCCACTAGCGCTCCCATTTCCTCCAGCTCCACCTTC A C BNO: 61 CTTATCC[C/A]CCTGTTTCCCCGTGGTCGTCCTCTCCCCTTC CACCTCCCTGTACTTCTGCSNP60 3 92.321 45626043 SEQ IDTTTGGCCATGGTGAGGGGGAAGCTGCAGGCGAAGAAGAAGGAG C T A NO: 62ACAGCTG[C/T]GGAGATTCGTCTGCAGGAGGTGCGAGCTCGG ATCAAAGCTTTGACCGAGT

TABLE 2G Location of SNP on SEQ Deposited SNP ID Chr cM chromosomeID NO: [SNP] and its adjacent sequences (5′ → 3′) A B line SNP61 3 93.347 47819830 SEQ ID CGGTTCGATCAGCTTGCACTTCTCGGTAAAGCAATGGATGTA G A ANO: 63 GAAGACCA[G/A]ATCGAATACATTGTTGAAGGCCTATCCGA TGATTACAAGCAGGTAGCTGASNP62 3  93.945 48535735 SEQ IDGCTTCAACACTAAGGTACTATAGAAGAACTTGTTTATGTATG T C B NO: 64TTGCATCT[C/T]CATACGTGTCTGCGCTTTGGATTCGGTCA ATCATTGTATGTATGTTGCAT SNP63 3 98.958 52628849 SEQ ID CTGTTTACCCAAGAACTTGATGCCAACGACCCTGTCTTTGTC T C BNO: 65 GTATGGGCT[C/T]AGGAGAAGAGGACATGGCCATGTTGCAC ACCACTTCGGTTTGGTAAGTGGSNP64 3  99.714 53167534 SEQ IDCATTATGTTCTGCAGCATCTGCAGTGGATCACCATGGCTCAC T C B NO: 66CACCAGAA[C/T]TGCGCACCTTTGAAATTCTGCTTCCATGG ATAACATGGCAGTGGCAAGTC SNP65 3109.082 58494494 SEQ ID TAAACTGTATTAGCTCACTTTCAGCATCGGTTAGTCCAGACG T G ANO: 67 AGAAGAAT[G/T]ACGACTTCTGAACACTTACTGCCAAGCCT GATCGAAGCTCAAATCCTTGASNP66 3 109.268 58569947 SEQ IDAGTCATAAGTCTTCTTCACTCTCTTTGTTACACGGTAGCTTA C A A NO: 68GAACAGCC[A/C]CAAGAAAAAGAACAAAGACAAAGAACGGC ACACAAATTGCTAAGATTATC SNP67 3109.415 58628362 SEQ ID AGAATTGGTCTGCAGCCAAGCAGATTCAAGCGATGGTGGCTA C T BNO: 69 ATCTTGTG[C/T]TCCCACGTGGAGCAGAAGCGATGCCGGTT TACATAATGAGCAGTGTTATGSNP68 3 113.736 60116952 SEQ IDCCTTCTCACTCGCTGCAGCGACTTCTCTTCTTCTTCATCACT C G B NO: 70AACAACAA[G/C]ACTAACAAACTCGTCAGCTTCTTCCTCAT CACCTCTCTCGGTTTCTTCAT SNP69 3117.120 61115979 SEQ ID TTTGACATTGGCTCATCAGGAGGTGGAGGTGGTGGGTCTGTT A G BNO: 71 CCATAGGGC[A/G]GTGATGAAACGGTCCAGACAGTTGGAGT GGAGGTGGTCTGTGTTGCAGACSNP70 3 121.679 62286307 SEQ IDACGTCCCTTTGTTGTCATCCCACCAGAATCGAGTGAATGCAG C T B NO: 72ACTGTATC[C/T]GTTTGCACAATGAAACCGGGAGCTTGAAG CAAGTCATAGAGTGCGATGGA

TABLE 2H Location of SNP on SEQ Deposited SNP ID Chr cM chromosomeID NO: [SNP] and its adjacent sequences (5′ → 3′) A B line SNP71 3125.279 63119854 SEQ ID AGCTTCCAAAGAACCTTCCCAAACCTAAACCATACCAAGAAT G A BNO: 73 CCAAGCAC[A/G]AAACCACCTAAAACACTCGACAGCAATGA TCTCCGACGGCACGAGAGAGASNP72 3 131.139 64347040 SEQ IDTTATGCTGTTTTAGCACTAGTGCTTGACCGAGCACGACCTAG C T B NO: 74AGCAGCGG[C/T]TTCTGAGGGGGCTAACCATCCTAGCTGCA GCCGCTGCCTTCAGTCAGGAGC SNP733 131.253 64369480 SEQ ID CGGTGAGCGTTGGGGTTGATGTCGGGTTGGATACAGAGCTTT G AA NO: 75 GAGCCAGG[A/G]ACGAACTTTCTCGCTTCTACAACGGTCTTGAGACCTGAGTCTACTCTGCA SNP74 4  11.174  1826660 SEQ IDAGACCTCGTGCTCCCCTCTGTCGTAGTTCACGTACACGGATT C T A NO: 76TCTTCTCC[C/T]GGAGTGCGTCGGAGGTCTTCGCTGCAGCT CGAGGACGATAGGCGCGGGAA SNP75 4 14.947  2437167 SEQ ID CCATCTGCGAAAGCAATCCCGAGCTCAGGCAGCTCCATGTTG T C ANO: 77 CTCTCCTC[C/T]ACACCAGACACGTTGAAGCAAGGGTCTAG GATCGGGAAGTCTTCAAACACSNP76 4  27.358  4981234 SEQ IDAGTGCGAAGGATGATGTTCTCAGGTACTGCATTATTTTCTTC G T B NO: 78TTTATTTA[G/T]TTCAGTGCACTACATGTTACTGCTTTCAC TTGCCTCATTTCATTATTTTT SNP77 4 28.164  5155338 SEQ ID AAAAAAACTGCAAAGTTCTATATGAAAGTTTGAAAGAAGAAC A T ANO: 79 ATATACCA[T/A]TTTGTTGTAGACTCTGAGTCTTTCCTTGA CAACTTCTGCAGTGTCATCAGSNP78 4  30.983  5756433 SEQ IDCTGCAGCAGTCCAACTCATTCCCCGCTAGAACTGGAGATCCC C T A NO: 80CAGGCTAC[C/T]TCAGCTGCAAGCAATCCAGGTGTCTCTGG AGGACAGAAGCCGTTTGTGCC SNP79 4 34.723  6571438 SEQ ID TACGAGATCATCCTCTCGAAACTCCCTTGCAAATGGCGCCCC C A BNO: 81 GCTTTGGA[A/C]CATGTCATGAAAATCCTCAACCGTTAGAT TCAAGGCACGCTGCTGCAGAASNP80 4  36.210  6888107 SEQ IDGAGAAGGAGGAAGCCTTGGAGAGGGTTGGGAGTTTGAGTGAG T G A NO: 82GAAGCTGA[T/G]AAGAGCGGGAAGAGAGCGGAGAACGCGAG GGAACAGCTAGGAGCAGCGCA

TABLE 2I Location of SNP on SEQ Deposited SNP ID Chr cM chromosomeID NO: [SNP] and its adjacent sequences (5′ → 3′) A B line SNP81 439.468  7584976 SEQ ID CAGCGAGTGGCAGCAAATCCTATAACCCGCGTATGCATCGTTG C T ANO: 83 TCAAGAGG[C/T]CATGACGGCGACTAAGAGCATCGGCGACTT TATTGGTTTTGTCGGACTGASNP82 4 42.615  8421224 SEQ IDCCTCATTTCAAAACTTTCCCATCTCTAGTCTTCAGCTCAGTGA A C B NO: 84CGATGAG[A/C]TGATCAGTGGCAGGAACGGTAAAGAGATGGA TGAGAGTCAGAGTCCAGAG SNP83 442.934  8529326 SEQ ID CATTACGGAACTTTGAAGAGGTACGAGTTTGAGATGCATAGTA A T ANO: 85 GAGTCTC[A/T]ATGGCTTGGATTCTTGGACTTGGGCCTAGCC TTGGGCTTGTTCGTGTTCCSNP84 4 44.097  8977099 SEQ IDCTGAGGAGTTTGGTAGTGTCGCTAAAGCTACTGATAGTGATCT G A A NO: 86TGATTTC[G/A]TTGTGGTTTCTCCTTCAAAGGCTATTGAGGA TGATAAGGATGCTAAGGTT SNP85 455.699 37635182 SEQ ID AGAAATCTTGTCACAATTATCAAACATTCGCAACACATTAGCA C T ANO: 87 GGAGATT[T/C]GCCAAGTGAGAAACTCGTTCATGTCGTTGAG AAGCTTCAATGCAAGCCACSNP86 4 63.882 44453699 SEQ IDTGCGGAGGCCTGTAGTTGCGGTTTTCCAAAGCGGCTGCAATGG G A B NO: 88CTCTTTC[G/A]ACGGATGCTGCTGATACGGATCTGGAGGTTC TAGAGGAAGGCTTTGGGCA SNP87 479.868 49190065 SEQ ID ACAACGTTGCTCTGCAGATAGTGATCATATATCTCAGACTTAT G T ANO: 89 TAGACTC[G/T]AGGATAGCGAGAGCTGCCTTTCTTCTTCAAG AACTCTGGAATCTCCACCGSNP88 4 79.868 49190123 SEQ IDCGAGAGCTGCCTTTCTTCTTCAAGAACTCTGGAATCTCCACCG A T A NO: 90AACCACC[A/T]TCTCTAAAGGAGGAAGAAGGTCTTCTTGTAG CTCCAGTTGAGGCAGTATC SNP89 482.873 49966272 SEQ ID CAAAAGTAGAAGCGAAAGCTATGATGATGATTACAAAAGTCTC A C ANO: 91 CATCCTT[C/A]TAAACAAAATCGGCACGTATAATTATCCTAG ATATGTGTATATATAAGTASNP90 4 89.350 51208004 SEQ IDGTTAAGGTGTGGCGGGTCCACGTCATCTCAGGGAACCGAAGCC A C A NO: 92ATGACTC[C/A]AAGGCAGATCATGGCCGTTGAAGATGGCAGA AGAAACTTGACGCCTGCAG

TABLE 2J Location of SNP on SEQ ID [SNP] and its adjacent sequencesDeposited SNP ID Chr cM chromosome NO: (5′ → 3′) A B line SNP91 4 89.44051222103 SEQ ID GAGGGCGGTAGATCTCTCAGATTCCTCTCTCCATCTCC T C A NO: 93CTCGTTTTCTTC[T/C]GTCTCTTCTGCTGGTGGTGAAG CAAATAGACACTCTAGGTCTCTTAGTGTSNP92 4 89.932 51294829 SEQ ID TTCGATCAGGGATCTGCAGATCAGAAGAGAGACGCATG CG B NO: 94 GAGCACGTGATA[G/C]TAGGAGGCGTTCATGATCTCCCAGGAAACCAGAACAGTGGAGAGATCGAG SNP93 4 92.074 51600428 SEQ IDAGCGATGGGAGCATCTTGTACCTTGATCTTCCCTCCAA T A A NO: 95CATGGTAGGATC[A/T]TTCTTGATGGGTTGGTTCGGC GTCGTGTTCAAAGCAGACATAACCAGAGTSNP94 4 92.790 51699975 SEQ ID TCTCCGTCTGGAGTCTCTGTCATGTCTCCGCTCTCGCC TC A NO: 96 GTCCTCCGGGGG[T/C]AACGGGATGTCGTCGATGGCGTGGCCGCAGCCGAACGTTCCTGCTCTGCA SNP95 4 92.972 51724947 SEQ IDAAAGTGCAACCATCATGCATATCGACTTTATGATCATC A G A NO: 97TTTGAGATGGTC[A/G]ACAAGGGTTTGAATATCACCG GTCACCGAACACTCGGAGCCAGCGTAGGGSNP96 4 97.323 52290476 SEQ ID CTCGGTGCTGCAGCAGGAGGCTGGATCAATGACTACTA GA A NO: 98 CGGACGTAAAAA[G/A]GCCACCATGTTTGCTGATGTTGTTTTCGCAGCTGGAGCAATCGTCATGGC SNP97 4 99.247 52528712 SEQ IDAGAATAGGGTTTACCTGCAACAAGATTCTCACAAGCTC A C B NO: 99CGTGCTTCCAAA[A/C]CGAGAAGCTTCAAGAAAGCAT TGGTTCACGTTATCCGCGCCTCCTTCCACSNP98 4 103.716 53075337 SEQ ID GTCAACCGTGTGGAGCTGCTAGCCAAGAAAATCACAGA GA B NO: 100 GCTTGGTTACTC[A/G]TGCTTCTATATCCATGCGAAGATGGCTCAAGACCACCGTAACAGGGTTTT SNP99 4 104.352 53152564 SEQ IDGACAACATCTCTCTGATCTCACCGTTCACAACGCTCTG G T B NO: 101CACATCAGCAAA[G/T]ACCTTAGCCCACAACGGCTTC TCAGAGACCGGTAAATCGAAAAGATCATTSNP100 5 4.966 1140114 SEQ ID GAATCTGTACATGTTCCTTTGTTTATATGGAGAACTCG A GA NO: 102 TCCTTCAGACCG[A/G]GGGATGTGGGGGAAAGGTGTCTCTACAGGTACTTTCTTCTGCAGAACTCG

TABLE 2K Location of SNP on SEQ ID [SNP] and its adjacent sequencesDeposited SNP ID Chr cM chromosome NO: (5′ → 3′) A B line SNP101 5 4.9761141506 SEQ ID ACGACCCTTGGGATCGGGATAAGGAACGACACTGCACG G T B NO: 103TAGCGATCTCTT[T/G]GTTGATTCGAGCTCACGGTAC GAGATCTTCGCGGCGGAGTATCTCGGCGCSNP102 5 7.820 1514669 SEQ ID AACAACCATCTCTGCAGGAAAGAGATAGAGAGACCATA G CB NO: 104 AGAACTGAACTT[C/G]ATATGCATAAACATTTACAAAGTACCTTCAGCAGCATCTCCACTACCGCT SNP103 5 7.820 1514715 SEQ IDACTTCATATGCATAAACATTTACAAAGTACCTTCAGCA G C B NO: 105GCATCTCCACTA[C/G]CGCTAGAACCATCAGCGTTGT CAATCTCCATAGCAGGCTGCTTAGGAGCTSNP104 5 15.375 2554344 SEQ ID TCACGGAGGGCAGTCGCAGTTGAATCATCTATTCCCAG TC B NO: 106 CAGATACTGTAG[T/C]CTCGACACCTTTTCTGGCGCCGGCTTGGGATCACTCTTTGAATAGATACT SNP105 5 15.608 2588838 SEQ IDGCGAAATGGACAGCAGTAAAAGGCGCGTTCATGAGAAC A G A NO: 107AGTCGTCCTATA[A/G]GAAGCGTAGAACGCACCAAAC CCTTCCTCCCTCATAACCCTCCTCACGCASNP106 5 16.607 2732567 SEQ ID CATAGAAAAGCCACGAGCGAAGCAACCTCTTCAGGCTC TC B NO: 108 CCCGAAACGCCC[T/C]AACGGCTTTCTAGACGTTACAGCCTTCTTGAAGTCATCATCGTATACCTG SNP107 5 31.035 4956729 SEQ IDCTATGATGATGTCCTCGCCCACTGTCTCCCAAGACGCT A G B NO: 109CGAATAAACTCA[A/G]CTGAGTAGCCATCCGGTCACG GGCTCTTGTTCAGTGGCATAGAGAATAATSNP108 5 41.841 10181899 SEQ ID ACCTGGACCACCACCATCTCCAGCGCCTACTGCAGAAA CT A NO: 110 CCACAGACACAT[C/T]GTCACCTGCCGCACCACAACAACAACCAACACGGATGAGCACCAGAAACC SNP109 5 44.362 12281717 SEQ IDTACCGTTAGTGCTCTGCTTCGGTTTATTCCTCAACTTT T A B NO: 111TGGCAAGCGTCA[A/T]GAGGATCAGCCACGTAAAGGA CTCCGGTTTCTACCGTAGCCTCTTCTGATSNP110 5 47.460 18521685 SEQ ID TATACGCAATCCTGCAGCATAATGAACATCAGTAGGAG GT A NO: 112 CTTGGAGAAACT[G/T]ACTCAGTCTGTGAACAGTAAAGGTTATATCTGGACGAGTAATAGTCAGAT

TABLE 2L Location of SNP on SEQ ID [SNP] and its adjacent sequencesDeposited SNP ID Chr cM chromosome NO: (5′ → 3′) A B line SNP111 548.732 30884244 SEQ ID AGTCATCGGGCGTGGACCATGTTAGCGAACTCGCGCTT G C BNO: 113 GTCTCTGCCTTG[G/C]ACTGACAAGATTTGATGGCGGAGACGGTATCGCAGCAGTATTGTGATCCT SNP112 5 51.345 37641346 SEQ IDCCTGCAGAAGGTGGAGACCAGACCACTCTCGAAGCTGA G C A NO: 114TGTTGTCCTCGT[G/C]TCAGCAGGAAGATCTCCCTTC ACATCTGGACTTGATCTTGACAAAATCGGSNP113 5 52.528 38794559 SEQ ID TCCCGAGTTGGCTGCAGGCGTACACCACCAGATGTAAC TC A NO: 115 AACTGTTTCCAG[C/T]AGCTTTTCCTCACGGGTTTTGCTCTCAAGACGAACTGAACCCGAACTTTC SNP114 5 56.319 41364962 SEQ IDCTGGTTTCGATGATCTACGAAAAGGGCTTGACACTTCC T C B NO: 116TTGTCACTCGAA[T/C]CAAGGACACACCAGCGGCGAG ATCATAAACCTCATGACGGTTGATGCAGASNPI15 5 56.778 41506661 SEQ ID TGTATCAGCCGCCTATCTGGAAAATATACTTGTCTCCT CT B NO: 117 AACAATCGCAGG[C/T]CTAATCGGAGACAGAAGAGGTGACAATAGATCTGTAACTTTCTCCTTGTA SNP116 5 68.679 43448077 SEQ IDTTGGAACTTATTACTGTGTTTTTTTGTTTCTGTGGGAG C T B NO: 118CAGGGATGAGAG[C/T]GTTACCAAAGCAGCGGTTGCA GCAATGGGTGATCTGGCAGATGTTGTAGGSNPI17 5 71.693 43800880 SEQ ID GCAGATCCTGGCTTCGGCGAACAATACACTGCCTCCAA CG B NO: 119 AGATAACGCCAG[C/G]GGATTACAGAGCTGAAGTGCGCCGAGGATTGCTGACTCCAAGAAGTTCTA SNP118 5 76.081 44280290 SEQ IDAATGTGTTTACTGTGCTGTGCTCGACGCTGAGCCATGC C T B NO: 120AGTGCGAACGGG[C/T]AAGAAAAGTCAAGGCACGTCT GAATCTGCAGCCAAGGAACCTGAGGAATCSNP119 5 76.505 44322768 SEQ ID ATCCCATCCGATTTGACGGAGACGCCGAGAGATTCATC TC A NO: 121 CTTCGTCGGCGT[T/C]GTCTTCTCCAAGCTCTCTTGATTGAAGAATCTCTGAAGACGAAATGGCGT SNP120 5 76.505 44322808 SEQ IDTCGTCGGCGTTGTCTTCTCCAAGCTCTCTTGATTGAAG C G A NO: 122AATCTCTGAAGA[C/G]GAAATGGCGTTCTGGTGGCC GCTGATCGTTCTCGCCTTCGCTTACGCGAT

TABLE 2M Location of SNP on SEQ ID [SNP] and its adjacent sequencesDeposited SNP ID Chr cM chromosome NO: (5′ → 3′) A B line SNP121 581.616 44870044 SEQ ID GCGGTGACGGTGACGCCGCCGCCGACAAGGCGGCCACC G A ANO: 123 GGGGAGGCCGCC[G/A]AAGAAGAAAACGCCGGCGGAGGAGGTGATGAAGCGGCAGCTGCAGTGTAG SNP122 5 86.439 45492393 SEQ IDGACAGAGAAGAGTGCCTGACCACGTGGAGAAGAGCGTA T C B NO: 124GACGAAAACGCT[C/T]GAAGGTGCGTTCCCTTTGTTT GCTTCAGCTATGAGATCCCCATAGCTGCASNP123 5 86.707 45530316 SEQ ID GGGATCAGGAGCAGTATCAGTTGCAACACCAGGCTCAT TG A NO: 125 GGTGAGGCACAA[G/T]ATCACGATGGAAATGATGGGGGAGATCAAGTTGATGAAGGTGAGGAAGGA SNP124 5 93.187 46733625 SEQ IDCCCAGTGCCGTCACCAAAGGATGGAAAGCCAATCCTGT C T B NO: 126TGAATTTGATTC[C/T]GTCTTCAACAAGAGCAGACAC ACGTTTGCTTATGGCAGTCCAGATATCATSNP125 6 4.372 724690 SEQ ID TGAAAACAGCATTCCACACTCACAACGGTCACTATGAA C TB NO: 127 TACCTAGTGTTG[C/T]CCTTCGGATTGTGCAATGCCCCATCGACATTCCAGGCGTTGATGAACTCT SNP126 6 6.800 1007519 SEQ IDTTTCTGAGGTCATACTGTCGATTGATGCTGAGGTCGGA A G B NO: 128TCTCCAGTGCTC[A/G]GCTCTCCAGATCCATGATGTT CATCTCTCTTTGCCATTCTGAGGTCGTCASNP127 6 22.962 2922287 SEQ ID TCGTAGTTTTCGTTCTGATCATCAAAACCGAAGAAGTC GC B NO: 129 TTGTATGGGAAG[G/C]GAGCTAGTCGTGAATGACGTGTCAAAGTCATCATCGTCGCCAAGGATTGT SNP128 6 24.373 3114174 SEQ IDAAGTGCATGGGCTAGCATCGTAGCTGCAGTTTCCGCCA G T B NO: 130TCAAGTTGTGTG[T/G]TTCGCCTTCAAGTACCCTTTG TCTTCTTGTTTCACTCTCCACCGGGTCTGSNP129 6 28.036 3627446 SEQ ID AGGAGAGTTTCGTGGTTTTGATGGACGTATCTGAGAGT AG B NO: 131 AAGCCTGCCTCC[A/G]GTCAAACGCAAGAGCAAAGGTATAACCGAACCATCATCGTTGTTCGCAAC SNP130 6 31.980 4189191 SEQ IDATACTTTGATGGTGCAAAGATTGAATGCAGAGGTATGG A C A NO: 132CTTTTTTAGTCC[C/A]GTCCCTTCTGGATTGAATAGA CTTTTAGCTACTGCTGCAGATTTGTTCAT

TABLE 2N Location of SNP on SEQ ID [SNP] and its adjacent sequencesDeposited SNP ID Chr cM chromosome NO: (5′ → 3′) A B line SNP131 639.058 5440585 SEQ ID GTTGTTTCGTGCAAATCTCACTGCTGCAGCTTGAGCAT A G ANO: 133 TCGTCATAGTCT[A/G]GGAAGGTTGAGTTGTTGTTGCAGTCATACCAAGGACCGGCGTCTTCTCTT SNP132 6 39.058 5440620 SEQ IDCATTCGTCATAGTCTAGGAAGGTTGAGTTGTTGTTGCA A T A NO: 134GTCATACCAAGG[A/T]CCGGCGTCTTCTCTTCTTGTC GCATAGCATTCAGATGGCATTCTTCTTGASNP133 6 43.889 6852182 SEQ ID TTCCCATCAACAACCTTTGTAGGATCAGCAAGCGCCGC GA A NO: 135 CTGAGCACCTTC[G/A]GAAGTCTTATACACAAACAAAGCAAACCCTCGTGACTTCCCAGTGACTTT SNP134 6 44.870 7272721 SEQ IDATAACAACAGATCCGTCTGCAGCTATTCCCCAATACAA G A B NO: 136ACTCTCAGCTCC[A/G]TCAGATCCTAGAGCTGCGAAA ACAGAGGAGGTCTGAGCATCATAGACAACSNP135 6 48.965 10618159 SEQ ID ATCTTGATCTCTCTGCAGATCTGGCCACGAACAGTGTC GA A NO: 137 ATCGTGGTTACC[G/A]TTGATTATCTTGAGTGCGTATACGCGGGAGGTTGGACGGTGGACTACTCT SNP136 6 50.138 13042773 SEQ IDATAGCAAACCTGTTGGTGTAGTACTGATCTTGACAATT C T B NO: 138ATAGCCAGCCAA[C/T]GTTCTGGGTCGACCAGACATA ACATCTTCAGCAGCTGCAGCCTCAACATTSNP137 6 50.525 13884163 SEQ ID AAATATGCGATCCGAATCCGAACGGATACCCGAACGTC TC B NO: 139 GACCCCTAGCTC[T/C]GAAGGACAATGAAAACCAATCGATTCAGCGGCATAGAGCCATAGAGGAAG SNP138 6 52.027 15217464 SEQ IDGCCATTCTCCATCGAATTGAACAAGGTCTCGTTATCGA G C A NO: 140TTTGGAGCCAGT[C/G]AGTCCTGATGAGGTCCCAGTC ACTGCTGCTGGTGCGTTGAAGTCATACAASNP139 6 55.973 18016782 SEQ ID TGCAGTGAGAGCCAATGATGAATGGGTGCCCTACTCAC GC A NO: 141 CATCTCAAGCTG[G/C]TGTGTCTGACACAAAAGCCCGGGGAATAGCCACACAGGTTGGTCTTACTG SNP140 6 66.709 24827566 SEQ IDGTAGAAAAGAGGGGAAGAGTATTTATGAGTGAGCAAAC A G B NO: 142ACCTATCTATCG[A/G]GATTACAAATTAGTAGGAATA AAGTGCTTGCCTTCCCAAAGTCTATATCT

TABLE 2O Location of SNP on SEQ ID [SNP] and its adjacent sequencesDeposited SNP ID Chr cM chromosome NO: (5′ → 3′) A B line SNP141 667.753 29368693 SEQ ID AACTTTTCTTGAGAAACAACCGCAAACACGAGCGGGAA G A BNO: 143 GAGTTGGTTTTC[A/G]GCATCAAACCCCGCGGCGACTAGCAGTTTTCCGGAGTGTTTACCGCAGTC SNP142 6 67.775 30981277 SEQ IDAAACATGAGACCACACTGCAGTAGAAATTGGCTTGCCC C G A NO: 144GCTAACGCTACT[C/G]CAAATAATTGATGCCATACCC AGACAGCTTTGCAGACCTCAGTCGAAAGASNP143 6 70.902 36429664 SEQ ID GCTTATGAGTTGTGTGTTTTTTTCTATCTCAGATAAGT GA B NO: 145 TGTATTGGGACC[A/G]TGCGGCTCATGTTGGAACAAACACACCAGGAGGACTGGTACTAGTAGCGT SNP144 6 71.169 36729257 SEQ IDCTGCTACACCCACCTCTTCATTCCCATGGGGTTGAAGT C G B NO: 146ACTCCTCCTTGG[C/G]CATCGAACCCGTTGCTGTGAG GCGGTGCTTGTGAGTTCATCTCTATCTGTSNP145 6 73.316 38879766 SEQ ID CTATCTGCAGGAGCCCTAATACTGACACGTGTAATCTC TG B NO: 147 AAAATATTCCAC[T/G]GCGAAGAAGTTGAGGTGACCCATTTTGCTGTTGATAGATTTGATGCGGCT SNP146 7 1.305 20364274 SEQ IDTTAGAACACCTGTGCTGCCGCACTGTGGTCAAGGCTTC A G A NO: 148TCTCAGAGCCAA[A/G]GCTTCCGCCATCAGAGGTGAG GCTACATACTCTGCTCGAGCTTGAAACTCSNP147 7 1.653 25133727 SEQ ID GCAGTATACTCTGGTATGGTGATACCTCTGTACTTTGC AC B NO: 149 AGGGCTTTCATC[A/C]GACAAAAGCTCTTTGATCGGTTCATAAACCGTGTCATTTGGAAACAGACT SNP148 7 3.260 29384441 SEQ IDTCATCGCTCACGTCACTGCTCGTGCTTCCTCTGTAGAT A G A NO: 150ACTGCTTTTGCC[G/A]CTCTCCATGAAATCAAGGCGG CTTCCACTAAGCTTGGCGCTTCCGCTCGTSNP149 7 12.311 33894835 SEQ ID TCGTTACCGGTCCGAGCGCCGTCGCTGGTTTCTTCCCG GA B NO: 151 GAGAATCTTCCA[G/A]GAGAAACTCCACGAACTTCCCAGGTGCGCCAATTTAGCCCAAGTGAAGCA SNP150 7 13.369 34371604 SEQ IDGAACGGTTAGTTTCTCCACTAATTTCTCCAAATTCAGA T C A NO: 152CACGGGTTTGGT[C/T]TTATTCAGACATGTGGTTTAG TCAACGTAAAGCTAAAATGGGTAAAAGAC

TABLE 2P Location of SNP on SEQ ID [SNP] and its adjacent sequencesDeposited SNP ID Chr cM chromosome NO: (5′ → 3′) A B line SNP151 713.825 34583372 SEQ ID TACTGCAGAGATGCGCAAAGCCGAGGTCACGTGAGGCC A G BNO: 153 AAGAACTTGATA[G/A]AGAAGCAGAGCCTAGCTCTTTTCGGTCCCGAGGGAAAGGAGGAAGAGAGC SNP152 7 25.291 38743951 SEQ IDGGGTTTAGTACAATAGCCTCTGCAGTTGGTTACCCTGT T C B NO: 154CCACTCTGAGTT[C/T]GCTGACCTCAAGACATACACG AATGGTGTGATAAAGCTTCGTGTTGTAGTSNP153 7 28.998 40014701 SEQ ID AACATGAGTGTCCTCTTCTCTCCGAGGAACCCGGATCG GT B NO: 155 TGTCCCGATTCC[G/T]ACACTGAGACGTTTCTTTGGTGACGATTATTACATCTGCAGGTTTCAGGT SNP154 7 37.842 43233659 SEQ IDCTGCAGTATATTTTTTGGAATCACACTCTGCAAATCTG A C A NO: 156ATCTTTCTTGTT[A/C]TCTTTCTCTATCCCCTAATCT AAAAGATACTATCAACGAAGCGAACTTGCSNP155 7 38.592 43345503 SEQ ID TCACTGCAGTGAGTCTCATAAAGTACCATTTTATTTTG GT A NO: 157 ATTACAGGCTGG[G/T]GTTCTGGCTGGAGATGTGAGCGACATTGTCCTTCTCGACGTGACGCCGCT SNP156 7 41.700 43818102 SEQ IDTCATCAAGTGGCACCATAAGAGGGTTCGCCTCATCCAT G A B NO: 158CTCTACATTCAT[G/A]TCTGAATCGTAATCTAGTTTC ATTTCTTCATCTCCATCACCCTCCTGCAGSNP157 7 43.584 44096110 SEQ ID ATTTTGAGATGTCTGGACTGTTTCATCCTGATCAGATC TA B NO: 159 CGGTTGGTGGCG[T/A]CTGAACAGGAGGAGCTGCCCGGCTTAGGTTGGTTGAGGAAGCTGCAGCAG SNP158 7 44.266 44191945 SEQ IDGGTAAGGCTTCTCGAGGAACCTCGTGTAGAGTGTAAGT C T A NO: 160GAGAAACGAGGG[C/T]TTGGGGTTTACTACTATTCTT GGACATTCGATGGACAGCATCTCCAATGASNP159 7 44.266 44191975 SEQ ID GTGTAAGTGAGAAACGAGGGCTTGGGGTTTACTACTAT AG A NO: 161 TCTTGGACATTC[G/A]ATGGACAGCATCTCCAATGAGTCCTTATCAATTCGTGGCTGCAGAAGAAG SNP160 7 46.334 44490415 SEQ IDAGCCACAAAGAACGAGAAATCACTTCTTTCCAGTGCTT A G B NO: 162GTGGGAGATTTG[G/A]GCATGGAGATACAGAGATCAG AAGCCACCTGATGTTTGCTCATGAGATGG

TABLE 2Q Location of SNP on SEQ ID [SNP] and its adjacent sequencesDeposited SNP ID Chr cM chromosome NO: (5′ → 3′) A B line SNP161 748.124 44794150 SEQ ID GTGAACATGCCATCCACTTCGCTGTCTCCTGCGGGTAC T C BNO: 163 CTCAAAAAGAAA[T/C]GTCGGACAATCAGGTTTCCCTCCGGAAACCACAAATGCTGGGTATGAAAG SNP162 7 54.996 46191816 SEQ IDTGAAACTCATATGGTAATATAATTTTTTTTCTTCCTCA C T B NO: 164TATTCATTCTGT[T/C]TCACTTTGAGGCTTGATGAAT GAGTGTCTTGTGACAGGTGGAACTAGAGGSNP163 7 61.162 47585593 SEQ ID ACACGCATTGGTAACCTCTCGTTCTGAACAGCGTGCGC TA A NO: 165 GCAAGCTTCTAC[T/A]GATAGCTTCCTACAGTCCATTAGCCTGCAGATTCTCTTCTTCTCGCTCTT SNP164 7 61.702 47714484 SEQ IDGGTGGTGGTGCTGCAGGAGGCGGCGGAGGTACGGTAGA C G A NO: 166GGGTGATGGAGG[G/C]ACAGCGAAAGGAGCTTTCTCC GTCGTCACAAAGCCTCGCTTCTTCAACCCSNP165 7 63.419 48124324 SEQ ID GTGCAATCAGCTGAGAAGTGACCTTGCTTGTAACAGTT AC A NO: 167 GTTGCAGAGTCT[C/A]AGGTCACCAGGAGGGAGGTGGCGAGCCGTGCAGTCTTTAGCTTGGTGTCC SNP166 7 63.806 48216530 SEQ IDATCCCGAGTACTTGACTCTCTGCCTGACCACGGTAGAC G A B NO: 168TTGATTCTCAGA[G/A]ATTTTTTGACGCCAGAGACAT GGTTCCCAATTATACAGAGTCATCTCCGCSNP167 8 0.016 148296 SEQ ID CAGGTTCCTGGAGGGACAACACTGTTGGAGCATTTGCA A GB NO: 169 AGGTAAAGTTTC[G/A]ATAGAAGAGAGTGTGATGAGTGCTGCAGCAGAAGCTGTGAGGGCAGCAAT SNP168 8 0.016 2426797 SEQ IDATGATTTCGGAAAAGAAGCAGAAGCTAGAGCCGAAGAG G C A NO: 170AAGGAGACAAAA[G/C]CGATGAGCTGGAGAAGGAAAT GGTAGTGACCTTCAAGACCTAAGTGATCASNP169 8 0.032 13520717 SEQ ID CGCCGAACCCTGATCAACCGAGATCGGTGCAGGAAGCC CT B NO: 171 ACCGTTGAGACG[C/T]GTCGTCCAATCTCTGACGGATCTCAGCCTCAATAAAAAGGGCTGCAGATG SNP170 8 2.152 18675613 SEQ IDTGACCGCAAGCACATCTGACATCTCGGCCCGTTGAGAG A G A NO: 172ATCCATATGGCG[G/A]ACTCGAATCTTGCCCCAACTG CAGCGCCTGTAACCCCACACATTTTTTAA

TABLE 2R Location of SNP on SEQ ID [SNP] and its adjacent sequencesDeposited SNP ID Chr cM chromosome NO: (5′ → 3′) A B line SNP171 8 4.12319739628 SEQ ID ACCACTCGTTTCCAATGACGCTATTCGTTCCTCGGACC T C B NO: 173ACACATCTGGAT[C/T]ACCGCACGCTGGAACAGCTCA ATAACTGGACGTGGCAGTTCTGTGTTATCSNP172 8 4.915 20071352 SEQ ID GCTCTACTCATCTGCTCCAGCCTCACCGCGCTAATGGG CG A NO: 174 AGGGAGACTAGT[G/C]GCAGCGCCAACGCTTTGGCCTGAGTTACTACCTCCTCCTGTTGTTAAACC SNP173 8 11.767 24841237 SEQ IDATATGTACTCCGGCGAGTGGAATTTAGTGAAATCTGAA G C B NO: 175TGTTGGGCAGGG[G/C]GAGGGCGGATGATATCCATGT AGCGAGGGAGGTAATAGCTGCAGATGAGGSNP174 8 13.753 26341786 SEQ ID TAGATGAAAGCCAAGGTAGAGAAAGGGCAATAAACCAA CG A NO: 176 GTATATGAGATC[C/G]AACAAGACGCTAGATATGAACTTGCTGAAGGAGCTGCAGCTCCTGGAATA SNP175 8 21.371 28667380 SEQ IDTTCTGCCAGTCACTTTATCATAGATAACCTACAAACAA T A A NO: 177AACCAAACCCCA[A/T]TTCATCATAAATTCAAAACAA AACCAAATCTCAAATTATCTTCAAAGACCSNP176 8 31.456 31835841 SEQ ID ACGAGGCCATGGTCAGAATCAGCTGCAGCTGGATCAGC CT A NO: 178 TGGTTACGTTGA[T/C]TGTCCAGCTGTATGTTCACGTAATCTTCCGTGTCGTCAATGTATTCCCTC SNP177 8 32.905 32719677 SEQ IDCATGTTCACTATTATCATTCTGCACGCTTGTTCCATCC T G B NO: 179TTAGCCACAGAT[T/G]CTACAACATTGTCATCACCTG TTTTAGCTTCTCTCTGCAGCAGAACAAGASNP178 8 35.366 33804788 SEQ ID AAGAAAGAAAAGCTCCTCGCCAGCTGAGGAGGAGGAAG CT A NO: 180 CAGCGGACCTTG[T/C]GGGGTACGTGTGCGGGGTCCTCGGGAGAGATCTATCGGAGGATCCTTGGC SNP179 8 40.549 35972043 SEQ IDATAATCGTTGCGGTGGCGGTTCCAGTTTCGCCGCCGGA T G A NO: 181CCAGCACTCGCC[T/G]AGGTTGGGACACTTAGCCTCC TCGCAGACGGTGTGAAGGTTAAGGTCTCTSNP180 8 45.635 36966286 SEQ ID CGTCGAACCTCGGTTTAGCACGCAACCAGTGGTACAAC TC B NO: 182 ACATCCAGCCAT[T/C]TCGGGAAGAAGAACCTCTCCATCAGATCAGCCATTAGGTGTATCGGCACT

TABLE 2S Location of SNP on SEQ ID [SNP] and its adjacent sequencesDeposited SNP ID Chr cM chromosome NO: (5′ → 3′) A B line SNP181 849.698 37687701 SEQ ID AATCCAGAAGTTGAAAAGAAAGCGAGATCTTTGGCTAT C G ANO: 183 AACCTCACTATC[C/G]GATCACACTCAACTCTGTTTTTGGCCCTCAACTGTCGCAGCCGGGCTTGT SNP182 8 53.908 38577262 SEQ IDAGGAAGCTAGAATATCTACACTTGTGGGATTTGCCTGA C A A NO: 184GCTGATGAGCAT[C/A]TACTGGAGTCCTTTGCCGTTT CCGTATTTGAGTCTGATCAATGTACAAAASNP183 8 62.287 40758807 SEQ ID GCGTGGAGAACATCTCCACCGAAGTCCTCAAGTCCTTC GA B NO: 185 AAAATCCGGCAC[A/G]ACTTTCTCAGCATTCTCTCCCGTAGCCACCACAAGCGTCCTGCAGATATA SNP184 8 62.374 40785808 SEQ IDTTGTCGATCAAGGAAGAGGACAGTCAAACTGAGCGGGG G T A NO: 186AGATGAAGATAG[T/G]TTTGATAATGAACAACCACCA AGTCCTCCTATGCATTTATCTGCAGGGCTSNP185 9 −0.030 313527 SEQ ID CTCCCGGTTGATCATGTTCTGAAGTTCCATGAATGCTT G CA NO: 187 GTTCCCATGGAA[G/C]CTCCTCCATCTCCTTTGGAATGGTTAGATACAATATATTCTTGGAAGAAC SNP186 9 0.104 330755 SEQ IDTGCTTCCTCCTTTCACTGCCGTTTTCCCTCGTTAGCGG C T B NO: 188TTACATCCTCCG[T/C]ACCCCCGCCGCTGAGAAACTT GTTGAAAGTCTCGGCGTCGGGAACAGTGCSNP187 9 0.183 340706 SEQ ID GAGAAGAAGAGCTAGAGGCAGGGTTTCCCAGAAGTGAC G AB NO: 189 TGTGAATCAGAA[G/A]AGCTGTACAAGTAAGCAGGTTGAAGCAAGGGTGCGTTTGAAGCAGGGATG SNP188 9 1.079 455157 SEQ IDTAAGTGAGACAGTTGTTACAGTTTTTCTCAGACAAGTC C T B NO: 190TGGCGTGCACTG[T/C]ACGGAAGCGAAGAATCTAGTG TAAGGTGGAGAAGCAGGACCATCTCCTTGSNP189 9 7.242 1254086 SEQ ID TTCTCCTTCTCACGTAACCGATCCTCTAAACGATCTTT G AB NO: 191 GGCTGATCTGAG[A/G]GAGAGGAGGTTTCTCTTCAGCTGCAGATTCTCGGTTTCTGCATCTATCAA SNP190 9 10.178 1639986 SEQ IDATTGAGGTGTCTATTCAGGGTGAGCAGTTCACCAAGAC C T B NO: 192CTTCAAAGTGGA[T/C]CTTTTGCCAAAGATTTATGAG ACACTACAGAAGTTGGTTGGGCTGTTGAA

TABLE 2T Location of SNP on SEQ ID [SNP] and its adjacent sequencesDeposited SNP ID Chr cM chromosome NO: (5′ → 3′) A B line SNP191 915.497 2355085 SEQ ID CTGCAGAATCAACAGTCTAAGCTGGTGTCTGAAGACCA C T ANO: 193 CATAAATCTCGA[C/T]AACAAACAAAACATAGCTACATATTTCTCTTACCCGACTAGTGTCTACAA SNP192 9 26.071 3745424 SEQ IDGGGAAAGAGCCTCTGGCTTAGCTAAAGACGTCATCATC C G B NO: 194TGCTCGAGCTGC[C/G]GTTTCAGTTCCTCGAGGTGGG ATAGAGTTTGTTTCACAGCCTCCATAGCTSNP193 9 30.177 4464952 SEQ ID TTCTTGTACATGATAACGTGGTGGCATCCATCTTCTTT CT A NO: 195 GGCTTGAGCCGC[T/C]TTCTCATTGGTCGAGACAGTTCCAATGACGGTAGCTCCAAGCGCATTTGC SNP194 9 32.759 5079336 SEQ IDCTCTACTCCAACCGCAACTGCAGTCTGCAAGAAACCAA A C A NO: 196CCTTTCAAGAAC[A/C]AAAACCTTACCAACAAGCATA GAAGAAGTGAAGGAGAGATGAAAGGACCTSNP195 9 41.524 7839999 SEQ ID TGAAGACGGTTACGTTGTGACTAAGCCTGGTACTACGA CA B NO: 197 AGACGAGCGTGG[C/A]TGGTGTGTTCGCTGCGGGAGATGTGCAAGATAAGAAGTACAGGCAGGCCA sNP196 9 45.092 9246116 SEQ IDGGAGGCGTTCTCTCCGCGCTTGCAACGTTCAGGATTCT C T B NO: 198TCAGGAGCCGCT[T/C]AGGAATTTTCCTGATCTGGTG TCAATGATGGCTCAGACTAAAGTGTCTCTsNP197 9 53.464 17031088 SEQ ID TAGCTTCTTAGGTCCACTTTTTGTGCACACTGCTCTTC TG B NO: 199 AAACAGGTGGCT[T/G]CATGTCCTCTTACTCCCTGGGCTCTTGCTTGTCTGTCTTTCTAGCTTGTT sNP198 9 58.238 42755363 SEQ IDTTTGACCCGCCGGAGACTTGACAAAAGGAACCAGGGCA G A A NO: 200GCCGAGGATGAT[G/A]ATCTCGTCTTCATCTGTTCTT TGCCTAAACCTGAGTTGAGTTTAGCTGGTsNP199 9 60.841 44984162 SEQ ID CTTCCGCAGAATCAGCTTTATAATGGTCATCAAGCAAC GA A NO: 201 ACAGCAGCATCA[G/A]GCACAACAAACATCTATGCATCATTTGCAACCCCAAGTAGTTTCGGGATC SNP200 9 69.310 48249783 SEQ IDATTGTAGTGACCATTTCCATCTCTCTCTTCACCATCAT C T B NO: 202CCCTCTTCTCTA[C/T]CCTTTTGTTGAAGACCCTAAC TTCTTCTTCAAGCAACAACATCCAAGTCA

TABLE 2U Location of SNP on SEQ ID [SNP] and its adjacent sequencesDeposited SNP ID Chr cM chromosome NO: (5′ → 3′) A B line SNP201 970.587 48542186 SEQ ID AAGAAGATCCAGTCGATGAAGGACAAGAATCTGAGGTC C T BNO: 203 GGAGATGGAGAC[C/T]GTCACCAGGGACGCGAGGAGGCTCGCGGTTTCGTACTGCAGGATTCACCT SNP202 9 73.370 49125336 SEQ IDTTCAATGCGCTTGCTATCAAGAGATTGAAGGAGATTCA C G B NO: 204GTGTTACCGTGG[C/G]GTTAGGCACATCCAAGGGTTG CCGTGTCGTGGACAGAGAACCAAGAACAASNP203 9 77.936 50007823 SEQ ID AAGGAAGCAGGCTTTGCACGAACGTCGGTTGGCACTTG GT A NO: 205 AACAAGATGTAC[T/G]AACCTTCTCTTGAGTTTTTGTGTTTCTATTCTCCAATTTTTATTCATAAT SNP204 9 82.039 50576467 SEQ IDTAACAAACGTACCTTACAAATGGCAAGGCAGGGTCAGC C T A NO: 206ACAGCGAGTTCC[C/T]GAAGGCAATCTATCCGCTGAA TTTGCAAGGGAGCTAGCAAGACCATTTTGSNP205 9 95.733 51845327 SEQ ID TCCTTTTCAGCTGTTTTAGCACTTCGTCCACGTCCCCT GT A NO: 207 TCCACGCCCTCT[G/T]CCTCTACCCCTTCCACTGGGTCCCACTTGCCCCGTCTCATGCTGCAGTGA SNP206 9 100.571 52317409 SEQ IDGTCACCTCTCAGCAGGAATCGATTCAAGAGCTCTACGC G A B NO: 208TGAGCTCGACGA[A/G]GAGAGAAACGCGGCTTCCACG GCTGCGAACGAGGCGATGTCTATGATACTSNP207 9 106.361 53047247 SEQ ID CCTACTATCCTAAGGTTAGTCTCGATGTTCCCAGCAACT C A NO: 209 CTTTCTTAGCAA[C/T]CCGGCTCTACCAATCAAGGCGGTGCTTGCCGCCTCGAACGAAGAATGCCA SNP208 9 111.615 54261547 SEQ IDTTAAACAACAGGGGAAGAGGTGGTTTTACGGGGCGGCC A T A NO: 210TCGTGGTGGTTT[A/T]GGCGGTGGTAATTTCCGAGGT GGTAGAGGAGGCAGGGGAGGTAGAGGAGGSNP209 9 112.468 54570013 SEQ ID GCATCTGACATCATTGTCGAGACAGCAGAGGCATTCCTG A A NO: 211 GCCAAAACTCGG[G/A]TCTGCACGGCTTGTCCTGGTTGACTTGAGCCATGGGTCGAAGATTCTGTC

<Progeny Line>

The broccoli plant of the present invention may be a progeny line of adeposited line. The progeny line may be a plant individual of a progenyline, a part of a plant individual of a progeny line, or a seed of aprogeny line.

In the present invention, the “progeny line” or the “progeny broccoliplant” (hereinafter collectively referred to as the “progeny line”) is aplant obtained from a broccoli plant of the deposited line or theprogeny line thereof. In the present invention, the progeny line may bea plant obtained from crossing the deposited line with another depositedline or another broccoli plant, or by crossing the deposited line with awild broccoli plant. The progeny line may be obtained, obtainable, orderived directly or indirectly through self-crossing and/orcross-pollination of the deposited line or a progeny line thereof. Also,the progeny line may be derived from a parental line obtained from thedeposited line using traditional breeding methods such as self-crossingand/or cross-pollination. Examples of the progeny line includeself-crossing progeny lines and first-generation hybrids F1 (hybridfirst-generation line, F1 hybrid). In obtaining the progeny line, thedeposited line may be used as a female parent, a male parent, or bothparents.

The term “crossing” as used in the present invention refers to thecrossing of two parent lines. The crossing may be “cross-pollination” or“self-pollination.” Cross-pollination refers to fertilization by thebinding of two gametes derived from different plants. Self-pollinationmeans that pollens migrate from the anther to the stigma of the sameplant. Self-pollination can also be referred to as self-crossing, forexample. The crossing may include backcrossing, which is one of thetraditional breeding methods.

The “backcrossing” is one of the traditional breeding techniques inwhich a breeder repeatedly backcrosses a progeny line of the hybrid toone of the parental lines and introduces a characteristic into a plantor a variety. A plant including the characteristic to be introduced maybe referred to as a donor plant, for example. A plant into which thecharacteristic is introduced may be referred to as a recurrent parent,for example. The backcrossing can be performed by crossing a donor plantwith a recurrent parent, thereby obtaining a first-generation hybrid F1(hybrid first-generation line, F1 hybrid). The progeny line having acharacteristic is then crossed with a recurrent parent. Then, bybackcrossing and/or self-crossing several generations, thecharacteristic of the donor plant can be introduced into the recurrentparent.

In the present invention, the progeny line may be regenerated from acell culture or a tissue culture derived from the deposited line, aprotoplast, or a part of a plant individual; may be obtained byself-crossing the deposited line; or may be obtained by producing seedsfrom the plant individual of the deposited line.

The term “regeneration,” as used in the present invention, refers to thedevelopment or vegetative propagation of a plant from a cell culture, atissue culture, or a protoplast.

The “tissue culture” or “cell culture” may be a composition includingthe same or different types of isolated cells or may be a collection ofcells that are organized into parts of a plant. Tissue cultures ofvarious tissues of broccoli plants and methods for regenerating plantsfrom the tissue cultures are well known, and reference can be made toReferences 3 to 5 below, for example.

-   Reference 3: Lise N. Hansen et al., Genetic analysis of protoplast    regeneration ability in Brassica oleracea, Plant Cell, Tissue and    Organ Culture, 1999, vol. 58, pp. 127-32.-   Reference 4: Ravanfar S. A. et al, Plant regeneration of Brassica    oleracea subsp. Italica (Broccoli) CV Green Marvel as affected by    plant growth regulators, African Journal of Biotechnology, 2009,    vol. 8, no. 11, pp. 2523-28.-   Reference 5: Jong Hee Kim et al., Callus Induction and Plant    Regeneration from Broccoli (Brassica oleracea var italica) for    Transformation, Journal of Plant Biology, September 2002, vol. 45,    no. 3, pp. 177-81.

The progeny lines may have desired characteristics. The progeny linesmay have “essentially all physiological and morphologicalcharacteristics of the deposited line” when they are cultivated in thesame cultivation conditions, for example. Specifically, the progeny linemay have a common characteristic with the deposited line. As a specificexample, 1 or more, 2 or more, 3 or more, 4 or more, 5 or more, 6 ormore, 7 or more, 8 or more, 9 or more, 10 or more, 11 or more, 12 ormore, or 13 or more characteristics of the progeny line match thecharacteristics of the deposited line. The progeny line may be a planthaving the main characteristic of the deposited line. The maincharacteristics are characteristics of Characteristic Nos. 9, 13, 21,and 36 in Tables 1A and 1B, i.e., the characteristics of (1) to (3) and(9). The progeny lines may be, for example, plants having the samecharacteristic as the deposited line, except for 13 or less, 12 or less,11 or less, 10 or less, 9 or less, 8 or less, 7 or less, 6 or less, 5 orless, 4 or less, 3 or less, 2 or less, or 1 characteristic, i.e., 13 orless, 12 or less, 11 or less, 10 or less, 9 or less, 8 or less, 7 orless, 6 or less, 5 or less, 4 or less, 3 or less, 2 or less, or 1characteristic may differ from the deposited line. The “characteristicdifferent from the deposited line” may be a main characteristic of thedeposited line or a characteristic other than the main characteristic ofthe deposited line, and is preferably a characteristic other than themain characteristic of the deposited line. The “characteristic differentfrom the deposited line” can be made, for example, by the introductionof a characteristic and/or introduction of a gene, which will bedescribed below. In the progeny lines, all the characteristics ofCharacteristic Nos. 1 to 38 may be the same as the deposited line.Examples of the characteristic different from the deposited linesinclude diamondback moth (Plutella xylostella) insect resistance, fallarmyworm (Spodoptera frugiperda) resistance, LSL (Long Shelf Life, staygreen, ethylene insensitive), and characteristics of coloring green evenunder weak light (characteristics of coloring green even in hiddenareas). Each characteristic can be introduced, for example, by crossingwith a known plant having a locus associated with each characteristic.

The progeny line may include a cell containing at least one set ofchromosomes derived from the deposited line. At least 6.25%, 12.5%, 25%,30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 80%, 85%, 90%, 95%, 96%,97%, 98%, or 99% of allele of the progeny line may be derived from thedeposited line. That is, the progeny line may have at least about 6.25%,12.5%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 80%, 85%, 90%,95%, 96%, 97%, 98%, or 99% genetic complement with the deposited line.

The “allele” is any one or more genes, and all of which are associatedwith a trait or characteristic of a broccoli plant. In a diploid cell ororganism, a pair of alleles of a given gene occupy the correspondinglocus on a pair of homologous chromosomes.

The genetic complement can be calculated, for example, by decoding amolecular marker or a base sequence, comparing it with a molecularmarker or a base sequence of a Takii 12, and calculating a concordancerate. Examples of the molecular marker include SNP markers, amplifiedfragment length polymorphism (AFLP) markers, restriction fragment lengthpolymorphism (RFLP) markers, microsatellite markers,sequence-characterized amplified region markers, and cleaved amplifiedpolymorphic sequence (CAPS) markers. Methods for analyzing genomes usingthe molecular markers are well known and widely published (e.g.,References 6 and 7 below). The base sequence can be decoded, forexample, by extracting a chromosome from the progeny line and sequencingthe chromosome. The proportion of allele derived from the deposited lineand the proportion of genetic complement may be estimated, for example,by the number of times of crossing. In this case, the proportion can beestimated from the number of times of crossing from the deposited line.As a specific example, when the number of times of crossing from thedeposited line is n, the proportion can be estimated to be, for example,(½)^(n)×100%.

-   Reference 6: Sinchan Adhikari et al., Application of molecular    markers in plant genome analysis: a review, The Nucleus, 2017, vol.    60, issue 3, pp. 283-97.-   Reference 7: Elcio P. Guimaraes et al., MARKER ASSISTED SELECTION    Current status and future perspectives in crops, livestock, forestry    and fish, 2007, Springer, pp. 29-49.

Preferably, the proportion of allele derived from the deposited line andthe proportion of genetic complement is, for example, an average valueof the proportion of a plurality of progeny lines. The “plurality” here,for example, is the number of individuals capable of subjectingstatistical examination, and, as a specific example, is 200 individualsor more, and preferably 200 to 1,000 individuals.

The progeny line may have an SNP derived from the deposited line. TheSNPs of the deposited line are the SNPs shown in Tables 2A through 2U.At least 6.25%, 12.5%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%,80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% of the SNPs of the progenyline may be derived from the deposited line, for example. That is, atleast about 6.25%, 12.5%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%,70%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% of the SNPs of theprogeny line may match the SNPs of the deposited line. In the presentinvention, if 50%, 55%, 60%, 65%, 70%, 80%, 85%, 90%, 95%, 96%, 97%,98%, or 99% or more of the SNPs of a target broccoli plant match theSNPs of the deposited line, for example, the target broccoli plant canbe determined (discriminated, estimated, appraised, or distinguished) tobe a progeny line of the deposited line. For example, the progeny linepreferably has at least one SNP selected from the group consisting ofSNP7, SNP8, SNP20, SNP21, SNP75, SNP78, SNP81, SNP84, SNP90, SNP142,SNP165, and SNP177 among the SNPs of the deposited line, and morepreferably has at least one SNP selected from the group consisting ofSNP78, SNP81, and SNP142 among the SNPs of the deposited line.

The progeny line may have, for example, a mutation or transgene. In thiscase, the progeny line has, for example, one or more characteristicsmodified. The progeny line can be produced, for example, by transferringa mutation or a transgene into the deposited line or a progeny linethereof. The mutations may be introduced artificially or may beintroduced naturally. The mutation may be, for example, achemical-induced mutation or a radiation-induced mutation. Also, themutation may be introduced, for example, by a molecular biologicalprocedure or a genome-editing technique (e.g., Reference 8 below). As tothe transgene, a method using Agrobacterium tumefaciens can be given.

-   Reference 8: Yanfei Mao et al., Gene editing in plants: progress and    challenges, National Science Review, 2019, vol. 6, pp. 421-37.

Examples of the one or more characteristics include diamondback mothinsect resistance, fall armyworm resistance, LSL (stay green, ethyleneinsensitive), and characteristics of coloring green even under weaklight (characteristics of coloring green even in hidden areas).

The “transgene” refers to a desired gene introduced into the genome of aplant, e.g., by a genetic engineering procedure or a traditionalbreeding method. The transgenes may be derived from the same species orfrom different species, for example. The transgene may be a gene havingthe same base sequence as or a different base sequence from the speciesfrom which it is derived, for example. In the latter case, a differentbase sequence can be prepared, for example, by performing codonoptimization, the addition of a transcription control factor such as apromoter, or the like, to the same base sequence. The transgene may havea translated region and an untranslated region.

<Haploid Plant and Doubled Haploid Plant>

The broccoli plant of the present invention may be a haploid plantand/or a doubled haploid plant that is obtained, obtainable, or inducedfrom the deposited line. The haploid plant and/or the doubled haploidplant of the deposited line may be used in a method for producing aparent line of the deposited line. In one embodiment, the presentinvention may provide a plant of a haploid plant and/or a doubledhaploid plant, a plant part of a haploid plant and/or a doubled haploidplant, or a seed of a haploid plant and/or a doubled haploid plant.

The doubled haploid plants can be produced by doubling chromosomes inhaploid plants or cells (e.g., Reference 9 below). As a specificexample, haploid pollens are cultured under predetermined conditions toform 1n chromosome plantlets. The chromosomes are then doubled bytreating plantlets with chemicals such as colchicine, for example. Thus,the cells of the plantlets have chromosomes of 2n (doubled haploids).Then, by growing the plantlets after the treatment, it is possible toobtain the doubled haploid plants and progeny lines.

-   Reference 9: Jim M. Dunwell, Haploids in flowering plants: origins    and exploitation, Plant Biotechnology Journal, 2010, vol. 8, pp.    377-424.

<Method for Producing Broccoli Plant>

As described above, the method for producing a broccoli plant of thepresent invention includes the step of crossing a first broccoli plantwith a second broccoli plant, wherein the first broccoli plant is thebroccoli plant of the present invention. The production method of thepresent invention is characterized in that the broccoli plant of thepresent invention is used for at least one of parents in the crossing,and other steps and conditions are not particularly limited.

Further, the method for producing a broccoli plant according to thepresent invention includes the step of self-crossing (self-pollinating)the broccoli plant according to the present invention. The productionmethod of the present invention is characterized in that the broccoliplant of the present invention is self-crossed, and other steps andconditions are not particularly limited.

According to the production method of the present invention, a progenyline of the deposited line can be produced. Regarding the productionmethod of the present invention, reference can be made to thedescription as to the broccoli plant of the present invention.

In the present invention, the crossing between the first broccoli plant(first parental line) and the second broccoli plant (second parentalline) may be, for example, crossing between the same individuals(regular self-pollination), crossing between individuals of a linemaintained in the same clonal individual or inbred line(quasi-self-pollination), or crossing between different individuals(cross-pollination). In the case of the regular self-pollination, one ofthe first parental line and the second parental line is a female organin the same individual and the other of which is a pollen in the sameindividual. The quasi-self-pollination may be, for example, a case wherethe S genotype related to self-incompatibility is the same, and thecross-pollination may be, for example, a case where the S genotyperelated to self-incompatibility is different. In crossing of broccoliplants, preferably the first parental line and the second parental lineare of different S genotypes and the crossing is cross-pollination.

In the present invention, the first parental line is the broccoli plantof the present invention, e.g., a broccoli plant deposited under theAccession No. FERM BP-22393 or a progeny line thereof.

The second parental line is not particularly limited, and any broccoliplant can be used. The second parental line may be, for example,taxonomically a broccoli plant of the same species or a broccoli plantof different species with the first parental line. The second parentalline may be, for example, the deposited line or the progeny line orother broccoli plant.

The production method of the present invention may further include thestep of growing a progeny line obtained in the crossing after thecrossing, for example. Growth conditions in the growing are generalgrowth conditions for broccoli plants.

The broccoli plant of the present invention can be obtained, forexample, by the production method of the present invention.

<Method for Producing a Seed of a Broccoli Plant>

The present invention provides a method for producing a broccoli seed.The method for producing a broccoli seed according to the presentinvention includes the steps of self-crossing the broccoli plant of thedeposited line or crossing the broccoli plant of the deposited line withanother broccoli plant, and optionally gathering (collecting orharvesting) the resulting seed. The method for producing a seed of thepresent invention may provide a plant, a plant part, or a seed bygrowing a seed of a broccoli plant.

The method for producing a seed of the present invention may be a methodfor producing a seed derived from the deposited line. In this case, themethod for producing a seed of the present invention may include thestep of (a) crossing a plant of the deposited line with another broccoliplant to produce a seed. The method for producing a seed of the presentinvention may further include the steps of (b) cultivating a broccoliplant from the seed obtained in step (a) to produce a broccoli plantderived from the deposited line, and (c) self-crossing the broccoliplant obtained in step (b) or crossing the broccoli plant obtained instep (b) with another broccoli plant to produce an additional broccoliplant derived from the deposited line. The method for producing a seedof the present invention may further include the step of (d) optionallyrepeating steps (b) and (c) one or more times to further produce abroccoli plant(s) derived from the deposited line. In this case, as thebroccoli plant was cultivated from the seed obtained from step (a) instep (b), an additional broccoli plant that has been obtained in step(c) can be used. The “one or more times” is, for example, one to 10times, three to seven times, or three to five times. The method forproducing a seed of the present invention may further include the stepof collecting or harvesting the seed. The method for producing a seed ofthe present invention may provide a seed produced by the above methodand a plant or a part of a plant individual obtained by growing theseed.

<Method for Producing a Hybrid Broccoli Plant>

The present invention provides a method for producing a hybrid broccoliplant. The method for producing a hybrid plant of the present inventionincludes the step of crossing the broccoli plant of the presentinvention with another broccoli plant. The method for producing a hybridplant of the present invention may include the step of collecting orharvesting the seed obtained by crossing. The method for producing ahybrid plant of the present invention may provide a seed produced by theabove method and a hybrid plant or a part of a hybrid plant individual.

<Method for Introducing a New Characteristic>

The present invention provides a method for introducing at least one newtrait or characteristic (hereinafter, together referred to as a“characteristic”) into the deposited line. The method for introducing acharacteristic of the present invention can also be referred to as, forexample, a method for producing a broccoli plant into which a newcharacteristic has been introduced. The method for introducing acharacteristic of the invention includes the steps of (a) crossing aplant of the deposited line with a broccoli plant having at least onenew characteristic to produce a progeny line, and (b) selecting aprogeny line having at least one new characteristic, for example. Themethod for introducing a characteristic of the present inventionincludes (c) crossing the progeny line with the deposited line toproduce a backcross progeny seed(s), and (d) selecting a backcrossprogeny having at least one new characteristic and having essentiallyall physiological and morphological characteristics of the depositedline, for example. In steps (b) and (d), selection of a progeny linehaving a new characteristic may be performed by detecting thecharacteristic or by detecting a gene or a molecular marker associated(linked) with the characteristic. Examples of the new characteristicinclude diamondback moth insect resistance, fall armyworm resistance,LSL (stay green, ethylene insensitive), and characteristics of coloringgreen even under weak light (characteristics of coloring green even inhidden areas).

The method for introducing a characteristic of the present invention mayinclude the step of (e) optionally repeating steps (c) and (d) one ormore times to produce a broccoli plant(s) having at least one newcharacteristic. In this case, in the method for introducing acharacteristic of the present invention, a backcross progeny that hasbeen selected in step (d) can be used as the progeny line in step (c). Abroccoli plant obtained or obtainable in step (e) may have essentiallyall physiological and morphological characteristics of the depositedline. Regarding the “essentially all physiological and morphologicalcharacteristics,” reference can be made to the description as to theprogeny line by replacing a “progeny line” with a “broccoli plantobtained or obtainable in step (e).” The “one or more times” is, forexample, one to 10 times, three to seven times, or three to five times.The method for introducing a characteristic of the present invention mayinclude the step of collecting or harvesting a seed. The method forintroducing a characteristic of the present invention may provide a seedproduced by the above method and a plant or a part of a plant individualobtained by growing the seed.

<Method for Transferring a Transgene>

The present invention provides a method for producing a plant derivedfrom a deposited line having at least one new trait or characteristic.The method for transferring a transgene of the present invention canalso be referred to as, for example, a method for producing a broccoliplant into which a new characteristic has been introduced.

The method for transferring a transgene of the invention includes thestep of transferring a mutation or a transgene that imparts at least onenew characteristic into a plant of a deposited line, for example. Thetransfer of a mutation or a transgene can be performed, for example, inthe same manner as the transfer of a mutation or a transgene in theprogeny line. A broccoli plant obtained or obtainable by thetransferring may have essentially all physiological and morphologicalcharacteristics of the deposited line. Regarding the “essentially allphysiological and morphological characteristics,” reference can be madeto the description as to the progeny line by replacing a “progeny line”with a “broccoli plant obtained or obtainable by the transferring.” Themethod for transferring a transgene of the present invention may includethe step of collecting or harvesting a seed. The method for transferringa transgene of the present invention may provide a seed produced by theabove method and a plant or a part of a plant individual obtained bygrowing the seed. Examples of the new characteristic include diamondbackmoth insect resistance, fall armyworm resistance, LSL (stay green,ethylene insensitive), and characteristics of coloring green even underweak light (characteristics of coloring green even in hidden areas).

<Broccoli Plant Regenerated Product and Regeneration Method>

The present invention provides a broccoli plant regenerated from a cellculture, a tissue culture, or a protoplast of a deposited line(hereinafter referred to as a “regenerated product”). The presentinvention may provide cell cultures or tissue cultures of regeneratablecells or protoplasts derived from broccoli plants of a deposited line.The cells, tissues, or protoplasts may be derived from tissues includingleaves, pollens, embryos, cotyledons, hypocotyls, meristematic cells,roots, root tips, anthers, flowers, seeds, or trunks.

The present invention provides a method of growth or propagation of abroccoli plant of a deposited line. The propagation of the broccoliplant of the deposited line may be a vegetative propagation of thebroccoli plant of the deposited line. In this case, the method forregenerating a broccoli plant of the present invention includes thesteps of (a) collecting a tissue that can be grown from a plant of adeposited line, (b) culturing the tissue to obtain a grown shoot, and(c) rooting the grown shoot to obtain a rooted plantlet, for example.The method for regenerating a broccoli plant of the present inventionmay further include (d) optionally growing a plant from a rootedplantlet. Regarding the method of vegetative propagation, for example,reference can be made to References 10 and 11 below. The regenerationmethod of the present invention may provide a plantlet, a plant, or apart of a plant individual regenerated (produced) by the methoddescribed above. The plant may have essentially all physiological andmorphological characteristics of the deposited line. Regarding the“essentially all physiological and morphological characteristics,”reference can be made to the description of the progeny line byreplacing a “progeny line” with a “regenerated plant.”

-   Reference 10: Habtamu Gudisa Megersa, Propagation Methods of    Selected Horticultural Crops by Specialized Organs: Review, Journal    of Horticulture, 2017, vol. 4, issue 2, 1000198.-   Reference 11: Nitish Kumar et al., In vitro Plant Propagation: A    Review, Journal of Forest Science, 2011, vol. 27, no. 2, pp. 61-72.

<Harvest and Processed Product of Broccoli Plant>

The present invention provides a harvest and/or a processed product of adeposited line or a progeny line. The harvest is a whole plant or a partof a plant individual, preferably including a flower head or a seed.

When the harvest is a flower head, the harvest may include a peduncleimmediately below the flower head, in addition to the flower head. Thelength of the peduncle to be harvested is, for example, about 1 to 20 cmand about 7 to 15 cm. If the harvest is a flower head, the flower headmay be a bundle of a plurality of flower heads.

The processed product includes any product obtained by treating thedeposited line or the progeny line. The treatment is not particularlylimited, and can be, for example, cutting, slicing, grinding, pureeing,drying, canning, bottling, washing, packaging, freezing and/or heating.In the deposited line or the progeny line, a plant or a part of a plantindividual used in the processed product is, for example, a flower head.The processed product may be, for example, a product obtained by washingand packaging the deposited line or the progeny line. The processedproduct may be contained, for example, in a container of any size orshape. Specific examples of the container include a bag, a box, and acarton. The cut may be, for example, a floret processing of cutting themain flower head into small flower heads.

The present invention may provide a container containing one or morebroccoli plants. The container contains a whole plant or a part of aplant individual, preferably a flower head.

The present invention may provide a method for producing a broccoliplant as a food (a method for producing a food). The method forproducing a food of the present invention includes the step ofcollecting or harvesting a whole plant or a part of a plant individualof the deposited line or the progeny line, preferably a flower head ofthe deposited line or the progeny line, for example. In addition, themethod for producing a food of the present invention includes the stepof cultivating a broccoli plant of the deposited line or the progenyline until it is matured.

<Method for Determining Genotype>

The present invention provides a method for determining or detecting agenotype of a deposited line or a progeny line. The method fordetermining a genotype of the invention includes the steps of (a)obtaining a nucleic acid sample from a deposited line or a progeny line,and (b) detecting a genome in the nucleic acid sample, for example. Instep (a), the method for preparing a nucleic acid from the depositedline or the progeny line can be performed using a general method forpreparing a nucleic acid from a tissue. In step (b), for example, apolymorphism and/or an allele in the genome in the nucleic acid sampleis detected. Detection of the polymorphism and/or allele can beperformed using, for example, SNP genotyping, AFLP detection, genomicDNA RFLP identification, genomic DNA CAPS detection, genomic DNA randomamplified polymorphic detection, polymerase chain reaction, DNAsequences, allele specific oligonucleotide probes, DNA microarrays, andthe like. The polymorphism and/or the allele may be detected, forexample, by sequencing the base sequence of the genome, or, as describedabove, by referring to the SNPs of the deposited lines. In step (b), oneor two or more polymorphisms and/or alleles in the genomic DNA may bedetected. The method for determining a genotype of the present inventionmay include the step of storing a detection result of a polymorphismand/or an allele in a computer-readable medium. The present inventionmay provide a computer-readable medium produced by such a method.

The method for determining a genotype of the present invention may beperformed on any broccoli plant (target broccoli plant) in place of thedeposited line or the progeny line, for example. In this case, themethod for determining a genotype of the present invention may furtherinclude the step of determining whether the target broccoli plant is theprogeny line on the basis of the result of step (b), for example. Thedetermination can also be said to be, for example, discrimination,estimation, appraisal, or distinguishability. The determination can bemade based on, for example, a concordance rate between the result ofstep (b) and the genotype of the deposited line.

EXAMPLES

The present invention will be described specifically below withreference to examples.

It is to be noted, however, that the present invention is by no meanslimited to embodiments described in the following examples.

Example 1

Broccoli plants of the deposited line were bred to examine their traitsand characteristics and to examine polymorphisms possessed by thedeposited line.

(1) Breeding of Deposited Line

In 2007, a broccoli line of stable cultivation with many leaves (made byTAKII & CO., LTD) was crossed with a broccoli line with relativelyvigorous growth in a low-temperature period and no anthocyanincoloration (made by TAKII & CO., LTD) to obtain F1 generation. In 2008,the F1 generation was self-crossed to obtain F2 generation. The obtainedF2 generation was selected by focusing attention on the stability ofgrowth, the shape of the flower head, the color of the flower head, andthe like, and the selected F2 generation was self-crossed. After that,selection and self-crossing were repeated in the same manner, therebyobtaining F7 generation. Since it was determined that the targetcharacteristic was fixed, breeding was terminated at the F7 generationin 2013.

One hundred strains of F7 were seeded on Aug. 2, 2013 at the testinglaboratory (Sato, Okubo-cho, Tahara-shi, Aichi Prefecture), and thencultivated to examine that there was no variation in characteristics inF7 and that the bred broccoli variety had uniformity and stability.Then, the F7 generation was self-crossed, and the seed of the F8generation obtained was deposited in NITE-IPOD under Accession No. FERMBP-22393.

(2) Characteristics of Deposited Line

The traits and characteristics of F7 plant individuals were evaluatedaccording to the Broccoli Variant Test Guideline published by the MAFF.The traits and characteristics of F7 plant individuals were alsoevaluated based on the criteria for Characteristic Nos. 40 to 42. Theresults are shown in Tables 3A through 3C below. It has been verifiedthat the deposited lines also exhibit the same characteristics.Photographs of the F7 line at the time of harvest are shown in FIGS. 6Aand 6B. FIG. 6A is a photograph showing the whole view of thecultivation area of F7, and FIG. 6B is a photograph showing the wholeplant of F7.

TABLE 3A Characteristic Measurement No. Characteristics Method NoteDeposited Line 1 Plant: number of stems Observation 1: one, 2: more thanone 1 2 Plant: height Measurement (cm) 3: short, 5: medium, 7: tall 5(45 cm) 3 Leaf: attitude Observation 3: semi-erect, 5: horizontal, 7:semi-pendulous 3 4 Leaf: length Measurement (cm) 3: short, 5: medium, 7:long 5 (48 cm) 5 Leaf: width Measurement (cm) 3: narrow, 5:medium, 7:broad 5 (19 cm) 6 Leaf: number of lobes Observation 3: few, 5: medium,7: many 7 7 Leaf blade: color Observation 1: green, 2: gray-green, 3:blue-green 2 8 Leaf blade: intensity of color Observation 3: light, 5:medium, 7: dark 5 9 Leaf blade: anthocyanin coloration Observation 1:absent, 9: present 1 10 Leaf blade: undulation of margin Observation 3:weak, 5: medium, 7: strong 5 11 Leaf blade: dentation of marginObservation 3: weak, 5: medium, 7: strong 3 12 Leaf blade: blisteringObservation 3: weak, 5: medium, 7: strong 3 13 Petiole: anthocyanincoloration Observation 1: absent, 9: present 1 14 Petiole: lengthObservation 3: short, 5: medium, 7: long 5 15 Head: length of branchingMeasurement (cm) 3: short, 5: medium, 7: long 3 (3 cm) 16 Head: sizeMeasurement 3: small, 5: medium, 7: large 5 (cm × cm) (10.5 cm × 13 cm)17 Head: weight Measurement (g) 3: light, 5: medium, 7: heavy 7 (440 g)18 Head: shape in longitudinal section Observation 1: circular, 2:transverse broad elliptic, 3: 2 transverse medium elliptic, 4:transverse narrow elliptic, 5: triangular

TABLE 3B Characteristic Measurement No. Characteristics Method NoteDeposited Line 19 Head: color Observation 1: cream, 2: green, 3:gray-green, 4: blue-green, 2 5: violet 20 Head: intensity of colorObservation 3: light, 5: medium, 7: dark 5 21 Head: anthocyanincoloration Observation 1: absent, 9: present 9 22 Head: intensity ofanthocyanin Observation 3: weak, 5: medium, 7: strong 3 coloration 23Head: knobbling Observation 3: fine, 5: medium, 7: coarse 3 24 Head:texture Observation 3: fine, 5: medium, 7: coarse 5 25 Head: firmnessObservation 3: loose, 5: medium, 7: firm 7 26 Head: conspicuousness ofspiral Observation 1: obscure, 2: obvious 1 pattern 27 Head: bractsObservation 1: absent, 9: present 1 28 Peduncle: length Measurement (cm)3: short, 5: medium, 7: long 3 (2.5 cm) 29 Peduncle: thicknessMeasurement (mm) 3: thin, 5: medium, 7: thick 7 (42 mm) 30 Peduncle:hardness Observation 3: soft, 5: medium, 7: hard 5 31 Peduncle: colorObservation 1: white, 2: light green, 3: green, 4: tinged with 3 purple32 Plant: secondary heads Observation 1: absent, 9: present 1 34 Flower:color Observation 1: white, 2: yellow 2 35 Flower: intensity of yellowcolor Observation 3: light, 5: medium, 7: dark 5 36 Time of harvestMeasurement (date) 3: early, 5: medium, 7: late 7 (December 13) 37 Timeof beginning of flowering Measurement (date) 3: early, 5: medium, 7:late 5 (March 10) 38 Male sterility Observation 1: absent, 9: present 1

TABLE 3C Charac- Measure- teristic ment Deposited No. CharacteristicsMethod Note Line 40 Leaf: number Observation 1: few, 9: many 9 41 Stem:length Observation 1: short, 9: long 1 42 Leaf: depth of lobesObservation 1: shallow, 9 9: deep

(3) SNP Marker of Deposited Line

The SNPs described in Tables 2A through 2U were analyzed for thedeposited lines and the commercial broccoli varieties described below.The results are shown in Tables 4A through 4G below. As shown in Tables4A through 4G below, it was found that SNP7, SNP8, SNP20, SNP21, SNP75,SNP78, SNP81, SNP84, SNP90, SNP142, SNP165, and SNP177 are depositedline-specific SNPs and that the progeny line can be identified bycombining one or more of these SNPs. It was verified that F7 has thesame SNPs. In addition, in Tables 4A through 4G, A represents ahomozygous type of SNP A, H represents a heterozygous type of SNP A andSNP B, B represents a homozygous type of SNP B, chr represents achromosome number, and cM represents centimorgan.

(Products of TAKII & CO., LTD) SHASTA, ERUDE, HAITSU SP, FOREST,TBR-449, CASTLE, GREEN FACE, MEGADOME (Products of SAKATA SEEDCORPORATION) PIXEL, SK9-099, GREEN CANNON, GRANDOME, HEARTLAND, DESTINY,Gypsy, Emerald Crown, Marathon, Avenger, Imperial (Products of BroleadCo., Ltd.) SUBARU, FIGHTER (Product of Nacos) SHIKIMIDORI 96 (Product ofMIKADO KYOWA SEED CO. LTD.) SPEED DOME 052 (Product of Seminis, Inc.)IRONMAN

TABLE 4A Location of SNPs on De- De- De- De- De- De- De- SNP C chromo-posited posited posited posited posited posited posited ID hr cM somes AB line line line line line line line SHASTA ERUDE HAITSUSP FORESTTBR-449 CASTLE SNP 1 0.990 454335 G T A A A A A A A B H H H H B 1 SNP 16.422 1264234 G C B B B B B B B B B B B B B 2 SNP 1 9.780 1783522 C T BB B B B B B B B B B B B 3 SNP 1 24.743 4314082 T G A A A A A A A A A H BH B 4 SNP 1 36.521 7889954 A C A A A A A A A B B B B B H 5 SNP 1 39.1788784702 T C B B B B B B B B H B B B B 6 SNP 1 40.958 9420949 C T A A A AA A A B H B B B H 7 SNP 1 46.689 11980318 A G A A A A A A A H H H B B B8 SNP 1 52.193 13607081 G C B B B B B B B H H A A B H 9 SNP 1 52.27213635739 C G B B B B B B B A A A A B A 10 SNP 1 56.649 17705704 A G B BB B B B B H B H A B A 11 SNP 1 58.660 22288471 G T A A A A A A A H A H HB H 12 SNP 1 75.912 41231290 T C B B B B B B B H H A H H H 13 SNP 177.423 41526625 C A A A A A A A A A A A H A B 14 SNP 1 82.329 42118296 GT A A A A A A A A A A A H H 15 SNP 1 87.796 42587881 C G B B B B B B B HH A H B H 16 SNP 1 88.361 42631525 C T A A A A A A A H B H H B B 17 SNP1 88.361 42631552 A G A A A A A A A H B H H B B 18 SNP 1 95.612 43132615T A B B B B B B B H A H H H A 19 SNP 2 3.410 717479 T A A A A A A A A BH H H B B 20 SNP 2 3.410 717482 T A A A A A A A A B H H H B B 21 SNP 28.829 1481256 A T B B B B B B B B B B B B B 22 SNP 2 32.758 3792354 G AB B B B B B B H B B H B H 23 SNP 2 49.203 8267341 A G A A A A A A A B BB B B B 24 SNP 2 49.415 8420520 A C B B B B B B B B B B B B B 25 SNP 249.704 8638214 T C A A A A A A A B B B B B H 26 SNP 2 51.821 11081737 GA A A A A A A A B B B B H H 27 SNP 2 55.999 27333228 G A A A A A A A A AH H A H A 28 SNP 2 66.589 45424768 A G A A A A A A A A H H A B A 29 SNPGREEN MEGA- SK9- GREEN HEART- Emerald Mara- SHIKIMI- SPEED IRON- ID FACEDOME PIXEL 099 CANNON GRANDOME LAND DESTINY Gypsy Crown thon AvengerImperial SUBARU FIGHTER DORI 96 DOME 052 MAN SNP H B H A A A B H A H A AH H H B H H 1 SNP B H H B B B B H B H B B B H B B H B 2 SNP H B B B B BB B B B B B B B B B B B 3 SNP A H A B B A B A A H H H B A A B A H 4 SNPB H H B H H B H H B H H B H B B H A 5 SNP B B H B H H B H H B H H H H BB H H 6 SNP B H B B B B B B B B B B B B B B B B 7 SNP B H H B B B B H HH B B H H H H H B 8 SNP A H H H H H A A B H H H H A H A H H 9 SNP A A HH H H A A H H H H H A H A H H 10 SNP A H B H B H A H H B H H H H B H H H11 SNP B H H H B H A A H H H H H A H H A H 12 SNP H A A B H B B H H H HB H H H H H A 13 SNP A B H A A A B B A H A A A H H B H A 14 SNP H B H BA H A H H B A H H H B H A H 15 SNP A A A H A H B A H H A H H A H H A H16 SNP H B H H H H B A B A H H H A B B H H 17 SNP H B H H H H B A B A HH H A B B H H 18 SNP H A B B H B A B H B B B B B H H B B 19 SNP H B B BB H B H H B H H B H B B H B 20 SNP H B B B B H B H H B H H B H B B H B21 SNP B B B B B B B B B B B B B B B B H H 22 SNP H H H H B B A H B H BB H H H A H B 23 SNP B H H A B B B H B B B B B H B H H B 24 SNP H B B BH H B H H B H H H H B B B H 25 SNP H H H H H H B A H H H H H A B H H H26 SNP B H H H H H B A H H H H H A B H H H 27 SNP A A H A A A A A H A HA H A A A A B 28 SNP A A H H A H A A H H H H H A H A A H 29

TABLE 4B Location of SNPs on De- De- De- De- De- De- De- SNP C chromo-posited posited posited posited posited posited posited ID hr cM somes AB line line line line line line line SHASTA ERUDE HAITSUSP FORESTTBR-449 CASTLE SNP 2 64.493 46653493 A T B B B B B B B H H H B A B 30SNP 2 74.571 49757428 A G A A A A A A A A H H A H A 31 SNP 2 77.90150407246 G A B B B B B B B A H A H A H 32 SNP 2 83.240 51346970 C T A AA A A A A A A A A H A 33 SNP 2 83.240 51347002 T G B B B B B B B B H H BH B 34 SNP 2 84.263 51516676 A G B B B B B B B H H H H B H 35 SNP 284.263 51516714 T G B B B B B B B H H H H B H 36 SNP 2 86.232 51834225 AG A A A A A A A A A A A H A 37 SNP 2 86.232 51834270 C T A A A A A A A AA A A H A 38 SNP 3 1.187 675632 A C B B B B B B B A B H H A H 39 SNP 32.498 948354 T C B B B B B B B B B B B B B 40 SNP 3 5.739 1516935 G A BB B B B B B H B B B A B 41 SNP 3 5.739 1516977 A T B B B B B B B H B B BA B 42 SNP 3 20.656 3369359 A G A A A A A A A A A H H H A 43 SNP 321.013 3409356 T G B B B B B B B H B B B H B 44 SNP 3 28.303 4315752 T GA A A A A A A A H A A A A 45 SNP 3 38.853 5932635 G A A A A A A A A A AA A H A 46 SNP 3 48.248 8135062 T G B B B B B B B H H A A A B 47 SNP 349.393 8510724 C T A A A A A A A A H H A H H 48 SNP 3 54.259 10912440 TG B B B B B B B A B B B B H 49 SNP 3 56.414 12711867 C A B B B B B B B BB B B B B 50 SNP 3 59.622 14684781 A T B B B B B B B H H H B H H 51 SNP3 68.614 20818643 T C B B B B B B B H H A A H A 52 SNP 3 71.069 22019198A G B B B B B B B B B H A H H 53 SNP 3 75.613 23508195 T G A A A A A A AA A A H A B 54 SNP 3 76.384 23746683 C T B B B B B B B H H A A B A 55SNP 3 76.582 23809957 T C A A A A A A A B H B B B B 56 SNP 3 79.75524764254 T G A A A A A A A A H B B H H 57 SNP 3 80.244 24921510 T C A AA A A A A H H B B H H 58 SNP 3 83.407 26569816 A C B B B B B B B B B B BH B 59 SNP GREEN MEGA- SK9- GREEN HEART- Emerald Mara- SHIKIMI- SPEEDIRON- ID FACE DOME PIXEL 099 CANNON GRANDOME LAND DESTINY Gypsy Crownthon Avenger Imperial SUBARU FIGHTER DORI 96 DOME 052 MAN SNP B B A H BH B B B A H H A B H B B H 30 SNP A A A H H H H A A H H H H A H A A H 31SNP H H A A H H A A A A H H A A A H H A 32 SNP A A H H A H A A B H H H HA H A A B 33 SNP B B H H B H B B A H H H H B H B B A 34 SNP B B H H B BA H B H B B H H B H H B 35 SNP B B H H B B A H B H B B H H B H H B 36SNP A H H H H H A A B H H H B A H A A H 37 SNP A H H H H H A A B H H H BA H A A H H38 SNP B A A H H H B H A A H H H H A A H H 39 SNP B H B B B BB B H B B B B B H H B B 40 SNP B B H H H H B B H H H H H B H B B H 41SNP B B H H H H B B H H H H H B H B B H 42 SNP H A H H H H A A H H H H HA H A A A 43 SNP B B A H H H B B A A A H H B H B B H 44 SNP A A A A A AA A A A A A A A A A A A 45 SNP A A H B B H A A B H H H H A H A A A 46SNP H B H A A H H B A H H H A B H B B B 47 SNP A B H H B H A A H H H H HA B A A H 48 SNP B H B B B B B B B B B B B B B B B B 49 SNP H B B B B BB B B B B B B B B B B B 50 SNP B H B B B B B B B B B B B B B B B B 51SNP B H H H H H A B A B H H H B H H B B 52 SNP B B H B H B A B H B H B BB H H B B 53 SNP A B H A A A B H A H A A H H H B H A 54 SNP H A H A B HA H H A B H A H H A A B 55 SNP H B B H H A B H B H H A H H B B H H 56SNP A H B H H A B H B H H A H H B B H A 57 SNP A H B H H A B H B A H A HH B H H A 58 SNP B H H H H H B B H H H H H B A H B B 59

TABLE 4C Location of SNPs on De- De- De- De- De- De- De- SNP C chromo-posited posited posited posited posited posited posited ID hr cM somes AB line line line line line line line SHASTA ERUDE HAITSUSP FORESTTBR-449 CASTLE SNP 3 92.321 45636043 C T A A A A A A A A A H H A A 60SNP 3 93.347 47819830 G A A A A A A A A A H H H A A 61 SNP 3 93.94548535735 T C B B B B B B B A B H H H H 632 SNP 3 98.958 52628849 T C B BB B B B B B B H H H H 63 SNP 3 99.714 53167534 T C B B B B B B B B B H HH B 64 SNP 3 109.268 58494494 T G A A A A A A A A A A A H A 65 SNP 3109.415 58569947 C A A A A A A A A H H H A H H 66 SNP 3 113.736 58628362C T B B B B B B B H H H B H H 67 SNP 3 117.120 60116952 C G B B B B B BB H B B B H B 68 SNP 3 121.679 61115979 A G B B B B B B B B H H B H B 69SNP 3 125.279 62286307 C T B B B B B B B A H H B B H 70 SNP 3 131.13963119854 G A B B B B B B B A H H B H H 71 SNP 3 131.253 64347040 C T B BB B B B B H H H B H B 72 SNP 3 11.174 64369480 G A A A A A A A A B H H AH A 73 SNP 4 109.268 1826660 C T A A A A A A A A H H A A A 74 SNP 414.947 2437167 T C A A A A A A A B B B B B B 75 SNP 4 27.358 4981234 G TB B B B B B B B B H A B H 76 SNP 4 28.164 5155338 A T A A A A A A A B BB B B B 77 SNP 4 30.983 5756433 C T A A A A A A A B B B B B B 78 SNP 434.723 6571438 C A B B B B B B B H H H A H H 79 SNP 4 36.210 6888107 T GA A A A A A A H B B B H H 80 SNP 4 39.468 7584976 C T A A A A A A A B BB B B B 81 SNP 4 42.615 8421224 A C B B B B B B B A A A A H A 82 SNP 442.934 8529326 A T A A A A A A A A H H B A H 83 SNP 4 44.097 8977099 G AA A A A A A A B H H B B H 84 SNP 4 55.699 37635182 C T A A A A A A A B BB H H H 85 SNP 4 63.882 44453699 G A B B B B B B B H B B B H B 86 SNP 479.868 49190065 G T A A A A A A A B H H A H H 87 SNP 4 79.868 49190123 AT A A A A A A A A H H A H A 88 SNP 4 82.873 49966272 A C A A A A A A A AH H H B H 89 SNP GREEN MEGA- SK9- GREEN HEART- Emerald Mara- SHIKIMI-SPEED IRON- ID FACE DOME PIXEL 099 CANNON GRANDOME LAND DESTINY GypsyCrown thon Avenger Imperial SUBARU FIGHTER DORI 96 DOME 052 MAN SNP A AA H A H A A A H A H H A H H A A 60 SNP A A A H A H A A A H A H H A H H AA 61 SNP H A H A H H B H H A B H A H A A H H 62 SNP B A A A H H B A H AH H H H A H H B 63 SNP B B H H B H B B H H H H H B B B B B 64 SNP A A BB A B A A H B B H H A H A A H 65 SNP A H H H A H A A H H H H H A H H H H66 SNP B H A H H H B B H H H H H B H B H H 67 SNP B B H H B H B B H H HH H B H H H H 68 SNP B B H H H H B B H H H H H B A H B H 69 SNP H A H HH B B B H H B B H H H A H B 70 SNP H A A A H H B H A A H H A H A A H H71 SNP B B H H H H B B H H H H H B A H B A 72 SNP A A H H H H A A H H HH H A B H A B 73 SNP A H A H H A A A A A A A A A A H A A 74 SNP H B B HH H B H H B H H H H B B B B 75 SNP H B B B A B A B B B B B H B B B B B76 SNP B B H B B H B A H B A H B H B B B A 77 SNP B B B B B B B B B B BB B B B B B B 78 SNP A H B B H B A B H B B B H B B H H B 79 SNP H H H HB H B H H B A H B H B B B A 80 SNP B B B B B B B B B B B B B B B B B BB81 SNP A A A A A A A A B A H A A A A A A H 82 SNP H A A H H A B A A A AA H A A A A A 83 SNP H H B H B B B B B B B B B B B B B B B84 SNP H B B BB B A B B B B B B B B B B B 85 SNP H H H B H B B B H B H B B B B B B B86 SNP H B B B H H A H B B H H B H B B H B 87 SNP A H H A H A A A H A HA H A A A A B 88 SNP A A H H H H B A B H H H H A H A H H 89

TABLE 4D Location of SNPs on De- De- De- De- De- De- De- SNP C chromo-posited posited posited posited posited posited posited ID hr cM somes AB line line line line line line line SHASTA ERUDE HAITSUSP FORESTTBR-449 CASTLE SNP 4 89.350 51208004 A C A A A A A A A B H B B B B 90SNP 4 89.440 51222103 T C A A A A A A A B H B B H B 91 SNP 4 89.93251294829 C G B B B B B B B A A A A B H 92 SNP 4 92.074 51600428 T A A AA A A A A B B B B B B 93 SNP 4 92.790 51699975 T C A A A A A A A B B B HH A 94 SNP 4 92.972 51724947 A G A A A A A A A B B B H H A 95 SNP 497.323 52290476 G A A A A A A A A H A H H A A 96 SNP 4 99.247 52528712 AC B B B B B B B B H H H A H 97 SNP 4 103.716 53075337 G A B B B B B B BH H H H A B 98 SNP 4 104.352 53152564 G T B B B B B B B H B B B B H 99SNP 5 4.966 1140114 A G A A A A A A A A H B H H A 100 SNP 5 4.9761141506 G T B B B B B B B B H A H H B 101 SNP 5 7.820 1514669 G C B B BB B B B H H B H H B 102 SNP 5 7.820 1514715 G C B B B B B B B H H B H HB 103 SNP 5 15.375 2554344 T C B B B B B B B H A A A A H 104 SNP 515.608 2588838 A G A A A A A A A H B B B B H 105 SNP 5 16.607 2732567 TC B B B B B B B A A A H A B 106 SNP 5 31.035 4956729 A G B B B B B B B HA H H H A 107 SNP 5 41.841 10181899 C T A A A A A A A H H H A B B 108SNP 5 44.362 12281717 T A B B B B B B B A A B B B B 109 SNP 5 47.46018521685 G T A A A A A A A B B H A H A 110 SNP 5 48.732 30884244 G C B BB B B B B B H B H H H 111 SNP 5 51.345 37641346 G C A A A A A A A B H HH B H 112 SNP 5 52.528 38794559 T C A A A A A A A H A A A H H 113 SNP 556.319 41364962 T C B B B B B B B A B B H H A 114 SNP 5 56.778 41506661C T B B B B B B B H B B H H A 115 SNP 5 68.679 43448077 C T B B B B B BB A B H A H H 116 SNP 5 71.693 43800880 C G B B B B B B B H A H B H B117 SNP 5 76.081 44280290 C T B B B B B B B H B B B B B 118 SNP 5 76.50544322768 T C A A A A A A A B B H H B H 119 SNP GREEN MEGA- SK9- GREENHEART- Emerald Mara- SHIKIMI- SPEED IRON- ID FACE DOME PIXEL 099 CANNONGRANDOME LAND DESTINY Gypsy Crown thon Avenger Imperial SUBARU FIGHTERDORI 96 DOME 052 MAN SNP H B B B B H B H B B H H H H B B B H 90 SNP H HH H B A B H H H A A H H H B B A 91 SNP A A H H A B B A A H B B B H H A AH 92 SNP B H B H H H B H H B H H H H B B B A 93 SNP H H H H B H A A B HH H H A H A H H 94 SNP H H H H B H A A H B H H H A H A H H 95 SNP B H AA H A A A A A A A H A A H H H 96 SNP H B H H B H A B H H H H H B H H B B97 SNP H H H H H H H B H H H H H B H H B B 98 SNP B H B B B B H B B B BB B B B B B B 99 SNP H A H H A B A A H H B B H H H A H B 100 SNP H B H HB A B H H H A A H H H B H A 101 SNP B B B B H B A B B B B B B B B B B B102 SNP B B B B H B A B B B B B B B B B B B 103 SNP A B H H B A A H A HA A H H H B H A 104 SNP B A H H A B B H H H B B H H H A H B 105 SNP A HH H B A B H A H A A A H H B H A 106 SNP A A B B H B A A H H B B H H H HA B 107 SNP H H H H H H A H H H H H H H H H H B 108 SNP B B B H H B B BA B B B H H H A B H 109 SNP A A B B B H A A B B H H B H B B A A 110 SNPB B B H B B A B B B B B H B B H B B 111 SNP H A H B B H B H H H H H H HB B A H 112 SNP H B H A H A A H H H A A H H H B H B 113 SNP H A H B H BA H B H B B B H H A H H 114 SNP H A H H H B A H B H B B H H H A H H 115SNP B H B B B H A H H B H H B H B B H B 116 SNP A H H B B B B B H B H BH B B B B H 117 SNP A H B H B H B H B B B H H H B B B B 118 SNP B A H HH H B H B A B H H H A A A H 119

TABLE 4E Location of SNPs on De- De- De- De- De- De- De- SNP C chromo-posited posited posited posited posited posited posited ID hr cM somes AB line line line line line line line SHASTA ERUDE HAITSUSP FORESTTBR-449 CASTLE SNP 5 76.505 44322808 C G A A A A A A A B B H H B H 120SNP 5 81.616 44870044 G A A A A A A A A H H A H H H 121 SNP 5 86.43945492393 T C B B B B B B B B H H B H B 122 SNP 5 86.707 45530316 T G A AA A A A A A H H A B A 123 SNP 5 93.187 46733625 C T B B B B B B B B H AB A B 124 SNP 6 4.372 724690 C T B B B B B B B H H B H H H 125 SNP 66.800 1007519 A G B B B B B B B H A H A H H 126 SNP 6 22.962 2922287 G CB B B B B B B H H H B H B 127 SNP 6 24.373 3114174 G T B B B B B B B B BH A H H 128 SNP 6 28.036 3627446 A G B B B B B B B B B H A H H 129 SNP 631.980 4189191 A C A A A A A A A A A A A A A 130 SNP 6 39.058 5440585 AG A A A A A A A A H H A H A 131 SNP 6 39.058 5440620 A T A A A A A A A AH H A H A 132 SNP 6 43.889 6852182 G A A A A A A A A A H B H H A 133 SNP6 44.870 7272721 G A B B B B B B B B B A B H B 134 SNP 6 48.965 10618159G A A A A A A A A A H H A H A 135 SNP 6 50.138 13042773 C T B B B B B BB H B H H B B 136 SNP 6 50.525 13884163 T C B B B B B B B H B H A B B137 SNP 6 52.027 15217464 G C A A A A A A A H H B H H A 138 SNP 6 55.97318016782 G C A A A A A A A H H H A H H 139 SNP 6 66.709 24827566 A G B BB B B B B H B H H B B 140 SNP 6 67.753 29368693 G A B B B B B B B H H HB H B 141 SNP 6 67.775 30981277 C G A A A A A A A B B B B B B 142 SNP 670.902 36429664 G A B B B B B B B B H H B H B 143 SNP 6 71.169 36729257C G B B B B B B B H H B B B H 144 SNP 6 73.316 38879766 T G B B B B B BB H B B B A B 145 SNP 7 1.305 20364274 A G A A A A A A A H H H H H H 146SNP 7 1.653 25133727 A C B B B B B B B B B B B B H 147 SNP 7 3.26029384441 A G A A A A A A A H B H A H H 148 SNP 7 12.311 33894835 G A B BB B B B B B B B B B B 149 SNP GREEN MEGA- SK9- GREEN HEART- EmeraldMara- SHIKIMI- SPEED IRON- ID FACE DOME PIXEL 099 CANNON GRANDOME LANDDESTINY Gypsy Crown thon Avenger Imperial SUBARU FIGHTER DORI 96 DOME052 MAN SNP B A H H H H B H B A B H H H A A A H 120 SNP B A A H A H B HA A H H H H A A H A 121 SNP B B H B H B B B H B H B B B B B H H 122 SNPA A H A H A A A B A H A A A A A A H 123 SNP B B H B H B B B A B A B B BB B B B 124 SNP H B B H B H A H H B H H H H H B H H 125 SNP A B B H H HA H H B H H H H H A H B 126 SNP A B H H H H B B H H H H A B H A H B 127SNP H B B H B H A H H B H H H H H B H H 128 SNP H B B H H H A H H B H HB H H B H H 129 SNP H A A A A A A A A A A A A A A A A A 130 SNP A A H HH H A A H H H H H A H A H A 131 SNP A A H H H H A A H H H H H A H A H A132 SNP H A H B B A A A B H A H B A A A A A 133 SNP B B H A H A B B B HH A H B H B B B 134 SNP H A H B H H A A H H H H H A H A A A 135 SNP B BB B B B B B B B B B H B B B H B 136 SNP H B B B B B B B B B B B B B B BH B 137 SNP H A H B B H A A H H H H H A H A H A 138 SNP A B B B H H A HH B H H H H H H H A 139 SNP H B B B B B B B H B B B H B B B B B 140 SNPH B H H H H B B H H H H H B H B B B 141 SNP B B B B B B B B B B B B B BB B B B 142 SNP H B H B B B B B A B A B H B B B B B 143 SNP H A H B B BB H B H B B H H B H H B 144 SNP B B H H B H B B A H H H H H H H B B 145SNP H A A A H A B A A A A A H A A H H H 146 SNP B H H B B B B H B H B BH H B B H B 147 SNP H H B H A A A H B H H A H H H A H H 148 SNP H B B BB B B B B B B B B B B B B B 149

TABLE 4F Location of SNPs on De- De- De- De- De- De- De- SNP C chromo-posited posited posited posited posited posited posited ID hr cM somes AB line line line line line line line SHASTA ERUDE HAITSUSP FORESTTBR-449 CASTLE SNP 7 13.369 34371604 T C A A A A A A A H B H H H B 150SNP 7 13.825 34583372 A G B B B B B B B H B B H H A 151 SNP 7 25.29138743951 T C B B B B B B B A A H H H A 152 SNP 7 28.998 40014701 G T B BB B B B B A A H B A B 153 SNP 7 37.842 43233659 A C A A A A A A A H B HH B B 154 SNP 7 38.592 43345503 G T A A A A A A A A H H H B H 155 SNP 741.700 43818102 G A B B B B B B B B A H H H A 156 SNP 7 43.584 44096110T A B B B B B B B A A B H H H 157 SNP 7 44.266 44191945 C T A A A A A AA A H A A A H 158 SNP 7 44.266 44191975 A G A A A A A A A B H H B B H159 SNP 7 46.334 44490415 A G B B B B B B B H B B B B H 160 SNP 7 48.12444794150 T C B B B B B B B H H H B B H 161 SNP 7 54.996 46191816 C T B BB B B B B B H H H H H 162 SNP 7 61.162 47585593 T A A A A A A A A A H HA H A 163 SNP 7 61.702 47714484 C G A A A A A A A B H A H B B 164 SNP 763.419 48124324 A C A A A A A A A B B H H B H 165 SNP 7 63.806 48216530G A B B B B B B B A A H H A H 166 SNP 8 0.016 148296 A G B B B B B B B HH B B B H 167 SNP 8 0.016 2426797 G C A A A A A A A H A H H H H 168 SNP8 0.032 13520717 C T B B B B B B B B H B B B B 169 SNP 8 2.152 18675613A G A A A A A A A A H A H B B 170 SNP 8 4.123 19739628 T C B B B B B B BB H H H B B 171 SNP 8 4.915 20071352 C G A A A A A A A H B H H H B 172SNP 8 11.767 24841237 G C B B B B B B B H A H B H H 173 SNP 8 13.75326341786 C G A A A A A A A B H A A B H 174 SNP 8 21.371 28667380 T A A AA A A A A H H H A B H 175 SNP 8 31.456 31835841 C T A A A A A A A B A AH B B 176 SNP 8 32.905 32719677 T G B B B B B B B A A A A A A 177 SNP 835.366 33804788 C T A A A A A A A A H B H A A 178 SNP 8 40.549 35972043T G A A A A A A A A A H A H A 179 SNP GREEN MEGA- SK9- GREEN HEART-Emerald Mara- SHIKIMI- SPEED IRON- ID FACE DOME PIXEL 099 CANNONGRANDOME LAND DESTINY Gypsy Crown thon Avenger Imperial SUBARU FIGHTERDORI 96 DOME 052 MAN SNP H B H B B H B H H B A H B H H B B H 150 SNP B AH A A H A H H A B H H H H A H H 151 SNP A A A A A H A H A A H H A H A AA A 152 SNP B B H H A B B B B B H B B B B B B H 153 SNP A B B H H A B HB H H A H H H B B H 154 SNP A A H A H A B A H A H A A A A A A A 155 SNPB A H H H B A H B H B B H H H A A H 156 SNP B B B B B B A B B B B B H BB B H B 157 SNP A H A A H A A A A A A A A A H H H A 158 SNP A H H H H HB A B H H H B A H A A A 159 SNP H H B B B B B B B B B B B B H H B H 160SNP B A H A H H B H H A B H H H A A H H 161 SNP B B B B B B A B B B B BB B B B B B 162 SNP A H B H H A A H H H H A H H A H B A 163 SNP H B B BB H B H B B H H B H B B B H 164 SNP H H B B B H B H H B H H B H B B H H165 SNP H H A A A H A H H A H H A H A A A H 166 SNP B H B A B H B B B HB H H B H A B H 167 SNP B B H H H H A B H H H H H B H B B H 168 SNP B BB H B H B B B H B H H B H B B B 169 SNP A H H B H H B H A B H H B H B BH A 170 SNP B H B H H H B B H H B H H B H B H H 171 SNP H H H B H H B HH B H H B H B B H A 172 SNP H H H H H H B B H H H H H B H B B H 173 SNPA B H H B H A A B H H H H A H H A A 174 SNP A H H H B H A A H H H H H AH A A A 175 SNP A B B B B H B H B B H H B H B B H B 176 SNP H A A A A HA H A A H H A H A A A A 177 SNP A H H H A A A H A H A A H H A A B A 178SNP A A H H H H A A B H H H H A H A A H 179

TABLE 4G Location of SNPs on De- De- De- De- De- De- De- SNP C chromo-posited posited posited posited posited posited posited ID hr cM somes AB line line line line line line line SHASTA ERUDE HAITSUSP FORESTTBR-449 CASTLE SNP 8 45.635 36966286 T C B B B B B B B A B B H A A 180SNP 8 49.698 37687701 C G A A A A A A A B H A H B B 181 SNP 8 53.90838577262 C A A A A A A A A A A A A H A 182 SNP 8 62.287 40758807 G A B BB B B B B H B B B B H 183 SNP 8 62.374 40785808 G T A A A A A A A H H AH H B 184 SNP 9 −0.030 313527 G C A A A A A A A H B B H A H 185 SNP 90.104 330755 C T B B B B B B B H A H B B H 186 SNP 9 0.183 340706 G A BB B B B B B B B H H B B 187 SNP 9 1.079 455157 C T B B B B B B B H B H HB B 188 SNP 9 7.242 1254086 G A B B B B B B B B B B H B A 189 SNP 910.178 1639986 C T B B B B B B B A H A H H H 190 SNP 9 15.497 2355085 CT A A A A A A A A B H H B H 191 SNP 9 26.071 3745424 C G B B B B B B B HB H H B H 192 SNP 9 30.177 4464952 C T A A A A A A A B H B B H B A93 SNP9 32.759 5079336 A C A A A A A A A A H H A A A 194 SNP 9 41.524 7839999C A B B B B B B B H B H B B H 195 SNP 9 45.092 9246116 C T B B B B B B BB B B B B H 196 SNP 9 53.464 17031088 T G B B B B B B B A B B H B H 197SNP 9 58.238 42755363 G A A A A A A A A A A A A H A 198 SNP 9 60.84144984162 G A A A A A A A A A A A H A H 199 SNP 9 69.310 48249783 C T B BB B B B B H B B B B H 200 SNP 9 70.587 48542186 C T B B B B B B B B B BB B H 201 SNP 9 73.370 49125336 C G B B B B B B B B H B B H B 202 SNP 977.936 50007823 G T A A A A A A A H A H B A B 203 SNP 9 82.039 50576467C T A A A A A A A A H B H A A 204 SNP 9 95.733 51845327 G T A A A A A AA B H H A H A 205 SNP 9 100.571 52317409 G A B B B B B B B H B H H B H206 SNP 9 106.361 53047247 T C A A A A A A A A H H A B A 207 SNP 9111.615 54261547 A T A A A A A A A B B A A B A 208 SNP 9 112.46854570013 G A A A A A A A A B H H H B A 209 SNP GREEN MEGA- SK9- GREENHEART- Emerald Mara- SHIKIMI- SPEED IRON- ID FACE DOME PIXEL 099 CANNONGRANDOME LAND DESTINY Gypsy Crown thon Avenger Imperial SUBARU FIGHTERDORI 96 DOME 052 MAN SNP B A A H H H A H A A H H A H A A A H 180 SNP A BB H H H B H H B H H B H B B B H 181 SNP H A B H H H A A H B H H H A H AA H 182 SNP H H H A H H B H A A B H A H A A H B 183 SNP A B A B A H B AA H A H H A B B H A 184 SNP H B H B A B A B H B H B H B B H B B 185 SNPB A H A B A B A H A H A H A A H A H 186 SNP H B B B B B B B B B B B B BB B B H 187 SNP H B B B B B B B B B B B B B B B B H 188 SNP B A H B B BA H B H B B H H H A H B 189 SNP A A A H H H B H A A H H A H H H H H 190SNP H A H B B B B H B H B B H H B H A B 191 SNP H H H B B B B H H H B BH H B H H B 192 SNP A B B H H A B H H H H A H H H B H H 193 SNP A A A AA A A A A A A A H A A A A A 194 SNP B B B B B B A B H B B B B B H H B H195 SNP B H B B B B B B H B B B B B B H B B 196 SNP B H H H H B A H H HB B B H H H H H 197 SNP A A H A A A A A H A H A A A A A A A 198 SNP A AH A A A B A B A A A H A A A H A 199 SNP B H B B B B A B B B B B B B B AH B 200 SNP B A B B B B B B B B B B B B B A H B 201 SNP H B B B H B B BB B B B H B B B B B 202 SNP A B H B H H B H H B A H H H B B B B 203 SNPA A A A A A A A A A A A A A A A A A 204 SNP H H H H A A A A B A H A H AA A A A 205 SNP H B B H B B B B H B B B H B B B H B 206 SNP H A H H H BA H H H B B H H A A A H 207 SNP B A H H H H A H H A B H H H A A A H 208SNP B A H A H H A H H A B H H H A A H B 209

While the present invention has been described above with reference toembodiments and examples, the present invention is by no means limitedthereto. Various changes and modifications that may become apparent tothose skilled in the art may be made in the configuration and specificsof the present invention without departing from the scope of the presentinvention.

SUPPLEMENTARY NOTES

Some or all of the above example embodiments and examples may bedescribed as in the following Supplementary Notes, but are not limitedthereto.

(Supplementary Note 1)

A broccoli plant including a broccoli plant identified by Accession No.FERM BP-22393 or a progeny line thereof

(Supplementary Note 2)

The broccoli plant according to Supplementary Note 1, wherein

the progeny line has 50% or more allele of the broccoli plant identifiedby Accession No. FERM BP-22393.

(Supplementary Note 3)

The broccoli plant according to Supplementary Note 1 or 2, wherein

the progeny line has 50% or more allele of the broccoli plant identifiedby Accession No. FERM BP-22393, and

the progeny line has the following characteristics (1) through (10):

(1) anthocyanin coloration of leaf blade: absent;(2) anthocyanin coloration of petiole: absent;(3) intensity of anthocyanin coloration of head: weak;(4) number of leaves: many;(5) length of stem: short;(6) firmness of head: firm;(7) number of lobes: many;(8) depth of lobe: deep;(9) time of harvest: late; and(10) self-incompatibility gene: SRK¹⁸ gene and SLG¹⁸ gene.

(Supplementary Note 4)

The broccoli plant according to any one of Supplementary Notes 1 to 3,wherein

the progeny line has 50% or more allele of the broccoli plant identifiedby Accession No. FERM BP-22393, and

the progeny line has at least one SNP selected from the group consistingof SNP7, SNP8, SNP20, SNP21, SNP75, SNP78, SNP81, SNP84, SNP90, SNP142,SNP165, and SNP177.

(Supplementary Note 5)

The broccoli plant according to any one of Supplementary Notes 1 to 4,wherein

the broccoli plant is a plant body or a part thereof

(Supplementary Note 6)

The broccoli plant according to any one of Supplementary Notes 1 to 5,wherein

the broccoli plant is a seed.

(Supplementary Note 7)

A method for producing a broccoli plant, including the step of:

crossing a first broccoli plant with a second broccoli plant, wherein

the first broccoli plant is the broccoli plant according to any one ofSupplementary Notes 1 to 6.

(Supplementary Note 8)

A seed of a broccoli variety Takii 12, wherein

a typical sample is a seed of a broccoli plant deposited under AccessionNo. FERM BP-22393.

(Supplementary Note 9)

A broccoli plant of a broccoli variety Takii 12, wherein

a typical sample is a seed of a broccoli plant deposited under AccessionNo. FERM BP-22393.

(Supplementary Note 10)

A broccoli plant or a part thereof, having essentially all physiologicaland morphological characteristics of the broccoli plant according toSupplementary Note 9.

(Supplementary Note 11)

A progeny broccoli plant of the broccoli plant according toSupplementary Note 9, having at least 50% allele of the broccoli plantaccording to Supplementary Note 9, wherein

the progeny broccoli plant has the following characteristics (1) through(10):

(1) anthocyanin coloration of leaf blade: absent;(2) anthocyanin coloration of petiole: absent;(3) intensity of anthocyanin coloration of head: weak;(4) number of leaves: many;(5) length of stem: short;(6) firmness of head: firm;(7) number of lobes of leaf: many;(8) depth of lobe of leaf: deep;(9) time of harvest: late; and(10) self-incompatibility gene: SRK¹⁸ gene and SLG¹⁸ gene.

(Supplementary Note 12)

A seed for production of the broccoli plant according to SupplementaryNote 11.

(Supplementary Note 13)

A progeny broccoli plant of the broccoli plant according toSupplementary Note 9, wherein

the progeny broccoli plant has at least 50% allele of the broccoliplant, according to Supplementary Note 9, and

the progeny broccoli plant has at least one SNP selected from the groupconsisting of SNP7, SNP8, SNP20, SNP21, SNP75, SNP78, SNP81, SNP84,SNP90, SNP142, SNP165, and SNP177.

(Supplementary Note 14)

A seed for production of the broccoli plant according to SupplementaryNote 13.

(Supplementary Note 15)

A plant part of the broccoli plant according to Supplementary Note 9.

(Supplementary Note 16)

The plant part according to Supplementary Note 15, wherein

the plant part includes microspores, pollens, ovaries, ovules, embryonicsacs, egg cells, cuttings, roots, trunks, leaves, cells, or protoplasts.

(Supplementary Note 17)

A method for producing a broccoli seed, including the step of:

self-crossing the broccoli plant according to Supplementary Note 9, orcrossing the broccoli plant according to Supplementary Note 9 withanother broccoli plant, and

collecting (gathering) a resulting seed(s).

(Supplementary Note 18)

A broccoli seed derived from a broccoli plant produced by the methodaccording to Supplementary Note 17.

(Supplementary Note 19)

A broccoli plant or a part thereof produced by growing the broccoli seedaccording to Supplementary Note 18.

(Supplementary Note 20)

The broccoli plant or the part thereof according to Supplementary Note19, having at least 50% allele of a broccoli variety Takii 12 whosetypical sample is a seed of a broccoli plant deposited under AccessionNo. FERM BP-22393, wherein

the broccoli plant or the part thereof has the following characteristics(1) through (10):

(1) anthocyanin coloration of leaf blade: absent;(2) anthocyanin coloration of petiole: absent;(3) intensity of anthocyanin coloration of head: weak;(4) number of leaves: many;(5) length of stem: short;(6) firmness of head: firm;(7) number of lobes: many;(8) depth of lobe: deep;(9) time of harvest: late; and(10) self-incompatibility gene: SRK¹⁸ gene and SLG¹⁸ gene.

(Supplementary Note 21)

The broccoli plant or the part thereof according to Supplementary Note19, having at least 50% allele of a broccoli variety Takii 12 whosetypical sample is a seed of a broccoli plant deposited under AccessionNo. FERM BP-22393, wherein

the broccoli plant or the part thereof has at least one SNP selectedfrom the group consisting of SNP7, SNP8, SNP20, SNP21, SNP75, SNP78,SNP81, SNP84, SNP90, SNP142, SNP165, and SNP177.

(Supplementary Note 22)

The broccoli plant or the part thereof according to Supplementary Note19, having essentially all physiological and morphologicalcharacteristics of a broccoli variety Takii 12 whose typical sample is aseed of a broccoli plant deposited under Accession No. FERM BP-22393.

(Supplementary Note 23)

The broccoli plant or the part thereof according to Supplementary Note20, wherein

one or more characteristics have been modified.

(Supplementary Note 24)

The broccoli plant or the part thereof according to Supplementary Note23, wherein

the modification is performed by mutagenesis.

(Supplementary Note 25)

A method for producing a seed of a broccoli plant derived from thebroccoli plant according to Supplementary Note 9, including the stepsof:

(a) crossing a broccoli variety Takii 12, which is a seed of a broccoliplant deposited under Accession No. FERM BP-22393, with another broccoliplant to produce a seed;

(b) growing a broccoli plant from the seed obtained in step (a) toproduce a broccoli plant derived from the broccoli variety Takii 12;

(c) self-crossing the broccoli plant obtained in step (b) or crossingthe broccoli plant obtained in step (b) with another broccoli plant toproduce an additional broccoli plant derived from the broccoli varietyTakii 12; and

(d) optionally repeating steps (b) and (c) one or more times to furtherproduce a broccoli plant(s) derived from the broccoli variety Takii 12,wherein the broccoli plant in step (b) has been grown from theadditional broccoli plant obtained in step (c).

(Supplementary Note 26)

A seed produced by the method according to Supplementary Note 25, havingat least 50% allele of the broccoli plant according to SupplementaryNote 9, wherein

the broccoli plant grown from the seed has the following characteristics(1) through (10):

(1) anthocyanin coloration of leaf blade: absent;(2) anthocyanin coloration of petiole: absent;(3) intensity of anthocyanin coloration of head: weak;(4) number of leaves: many;(5) length of stem: short;(6) firmness of head: firm;(7) number of lobes: many;(8) depth of lobe: deep;(9) time of harvest: late; and(10) self-incompatibility gene: SRK¹⁸ gene and SLG¹⁸ gene.

(Supplementary Note 27)

A broccoli plant produced by growing the seed of the broccoli plantaccording to Supplementary Note 26.

(Supplementary Note 28)

A seed produced by the method according to Supplementary Note 25, havingat least 50% allele of the broccoli plant according to SupplementaryNote 9, wherein

the broccoli plant grown from the seed has at least one SNP selectedfrom the group consisting of SNP7, SNP8, SNP20, SNP21, SNP75, SNP78,SNP81, SNP84, SNP90, SNP142, SNP165, and SNP177.

(Supplementary Note 29)

A broccoli plant produced by growing the seed of the broccoli plantaccording to Supplementary Note 28.

(Supplementary Note 30)

A method for introducing at least one new characteristic into thebroccoli plant according to Supplementary Note 9, including the stepsof:

(a) crossing a broccoli variety Takii 12, which is a seed of a broccoliplant deposited under Accession No. FERM BP-22393, with a broccoli planthaving at least one new characteristic to produce a progeny(s);

(b) selecting a progeny having at least one new characteristic;

(c) crossing the progeny with the broccoli variety Takii 12 to produce abackcross progeny(s);

(d) selecting a backcross progeny having at least one new characteristicand having essentially all physiological and morphologicalcharacteristics of the broccoli variety Takii 12; and

(e) optionally repeating steps (c) and (d) one or more times to producea broccoli plant(s) having at least one new characteristic and havingessentially all physiological and morphological characteristics of thebroccoli variety Takii 12, wherein the broccoli plant in step (c) is abackcross progeny that has been selected in step (d).

(Supplementary Note 31)

A broccoli plant produced by the method according to Supplementary Note30.

(Supplementary Note 32)

A method for producing a broccoli plant derived from a broccoli varietyTakii 12 having at least one new characteristic, including the step of:

transferring a mutation or a transgene that imparts at least onecharacteristic into a broccoli variety Takii 12, which is a seed of abroccoli plant deposited under Accession No. FERM BP-22393.

(Supplementary Note 33)

A broccoli plant produced by the method according to Supplementary Note32.

(Supplementary Note 34)

A method for producing a flower head of a broccoli as a food, includingthe step of:

harvesting a flower head or the flower head and a peduncle of thebroccoli plant according to Supplementary Note 9.

(Supplementary Note 35)

A processed product of the broccoli plant according to SupplementaryNote 9, including:

a cut, sliced, ground, pureed, dried, canned, bottled, washed, packaged,frozen and/or heat-treated flower head.

(Supplementary Note 36)

A method for determining a genotype of the broccoli plant according toSupplementary Note 9 or a progeny line thereof, including the steps of:

(a) obtaining a nucleic acid sample from the broccoli plant according toSupplementary Note 9 or a progeny line thereof, and

(b) detecting a polymorphism in the nucleic acid sample.

(Supplementary Note 37)

A tissue culture of regenerable cells or protoplasts derived from thebroccoli plant according to Supplementary Note 9.

(Supplementary Note 38)

The culture tissue according to Supplementary Note 37, wherein

the cells or protoplasts are derived from leaves, pollens, embryos,cotyledons, hypocotyls, meristematic cells, roots, root tips, anthers,flowers, seeds, or stems.

(Supplementary Note 39)

A broccoli plant regenerated from the tissue culture according toSupplementary Note 38.

(Supplementary Note 40)

The broccoli plant according to Supplementary Note 39, havingessentially all physiological and morphological characteristics of abroccoli variety Takii 12, which is a seed of a broccoli plant depositedunder Accession No. FERM BP-22393.

(Supplementary Note 41)

A method for vegetative propagation of the broccoli plant according toSupplementary Note 9, including the steps of:

(a) collecting a propagatable tissue from a broccoli plant of a broccolivariety Takii 12, which is a seed of a broccoli plant deposited underAccession No. FERM BP-22393;

(b) culturing the tissue to obtain a grown shoot;

(c) rooting the grown shoot to obtain a rooted plantlet; and

(d) optionally growing a plant from the rooted plantlet.

(Supplementary Note 42)

A broccoli plantlet or a plant produced by the method according toSupplementary Note 41, having essentially all physiological andmorphological characteristics of a broccoli variety Takii 12, which is aseed of a broccoli plant deposited under Accession No. FERM BP-22393.

(Supplementary Note 43)

A broccoli plant including a broccoli plant identified by Accession No.FERM BP-22393.

(Supplementary Note 44)

A broccoli plant including a progeny line of the broccoli plantaccording to Supplementary Note 43, wherein

the progeny line has the following characteristics (1) through (10):

(1) anthocyanin coloration of leaf blade: absent;(2) anthocyanin coloration of petiole: absent;(3) intensity of anthocyanin coloration of head: weak;(4) number of leaves: many;(5) length of stem: short;(6) firmness of head: firm;(7) number of lobes: many;(8) depth of lobe: deep;(9) time of harvest: late; and(10) self-incompatibility gene: SRK¹⁸ gene and SLG¹⁸ gene.

(Supplementary Note 45)

A broccoli plant including:

a hybrid first-generation line of the broccoli plant according toSupplementary Note 43 or 44.

(Supplementary Note 46)

The broccoli plant according to any one of Supplementary Notes 43 to 45,wherein

the broccoli plant is a plant body or a part thereof

(Supplementary Note 47)

The broccoli plant according to any one of Supplementary Notes 43 to 46,wherein

the broccoli plant is a seed.

(Supplementary Note 48)

A method for producing a broccoli plant, including the step of:

self-crossing the broccoli plant according to any one of SupplementaryNotes 43 to 47.

(Supplementary Note 49)

A method for producing a broccoli plant, including the step of:

crossing the broccoli plant according to any one of Supplementary Notes43 to 47 with another broccoli plant.

(Supplementary Note 50)

The method for producing a broccoli plant according to SupplementaryNote 48 or 49, including the step of:

gathering a seed(s).

INDUSTRIAL APPLICABILITY

As described above, according to the present invention, a new broccoliplant can be provided. For this reason, the present invention isextremely useful in an agricultural field such as breeding, for example.

1. A broccoli plant comprising: a broccoli plant identified by Accession No. FERM BP-22393 or a progeny line thereof.
 2. The broccoli plant according to claim 1, wherein the progeny line has 50% or more allele of the broccoli plant identified by Accession No. FERM BP-22393.
 3. The broccoli plant according to claim 1, wherein the progeny line has 50% or more allele of the broccoli plant identified by Accession No. FERM BP-22393, and the progeny line has the following characteristics (1) through (10): (1) anthocyanin coloration of leaf blade: absent; (2) anthocyanin coloration of petiole: absent; (3) intensity of anthocyanin coloration of head: weak; (4) number of leaves: many; (5) length of stem: short; (6) firmness of head: firm; (7) number of lobes: many; (8) depth of lobe: deep; (9) time of harvest: late; and (10) self-incompatibility gene: SRK¹⁸ gene and SLG¹⁸ gene.
 4. The broccoli plant according to claim 1, wherein the progeny line has 50% or more allele of the broccoli plant identified by Accession No. FERM BP-22393, and the progeny line has at least one SNP selected from the group consisting of SNP7, SNP8, SNP20, SNP21, SNP75, SNP78, SNP81, SNP84, SNP90, SNP142, SNP165, and SNP177.
 5. The broccoli plant according to claim 1, wherein the broccoli plant is a plant body or a part thereof.
 6. The broccoli plant according to claim 1, wherein the broccoli plant is a seed.
 7. A method for producing a broccoli plant comprising the step of: crossing a first broccoli plant with a second broccoli plant, wherein the first broccoli plant is the broccoli plant according to claim
 1. 8. A seed of a broccoli variety Takii 12, wherein a typical sample is a seed of a broccoli plant deposited under Accession No. FERM BP-22393.
 9. A broccoli plant of a broccoli variety Takii 12, wherein a typical sample is a seed of a broccoli plant deposited under Accession No. FERM BP-22393.
 10. A broccoli plant or a part thereof, having essentially all physiological and morphological characteristics of the broccoli plant according to claim
 9. 11. A progeny broccoli plant of the broccoli plant according to claim 9, having at least 50% allele of the broccoli plant according to claim 9, wherein the progeny broccoli plant has the following characteristics (1) through (10): (1) anthocyanin coloration of leaf blade: absent; (2) anthocyanin coloration of petiole: absent; (3) intensity of anthocyanin coloration of head: weak; (4) number of leaves: many; (5) length of stem: short; (6) firmness of head: firm; (7) number of lobes of leaf: many; (8) depth of lobe of leaf: deep; (9) time of harvest: late; and (10) self-incompatibility gene: SRK¹⁸ gene and SLG¹⁸ gene.
 12. A seed for production of the broccoli plant according to claim
 11. 13. A progeny broccoli plant of the broccoli plant according to claim 9, wherein the progeny broccoli plant has at least 50% allele of the broccoli plant according to claim 9, and the progeny broccoli plant has at least one SNP selected from the group consisting of SNP7, SNP8, SNP20, SNP21, SNP75, SNP78, SNP81, SNP84, SNP90, SNP142, SNP165, and SNP177.
 14. A seed for production of the broccoli plant according to claim
 13. 15. A plant part of the broccoli plant according to claim
 9. 16. The plant part according to claim 15, wherein the plant part includes microspores, pollens, ovaries, ovules, embryonic sacs, egg cells, cuttings, roots, trunks, leaves, cells, or protoplasts.
 17. A method for producing a broccoli seed, comprising the steps of: self-crossing the broccoli plant according to claim 9 or crossing the broccoli plant according to claim 9 with another broccoli plant, and collecting a resulting seed(s).
 18. A broccoli seed derived from a broccoli plant produced by the method according to claim
 17. 19. A broccoli plant or a part thereof produced by growing the broccoli seed according to claim
 18. 20. The broccoli plant or the part thereof according to claim 19, having at least 50% allele of a broccoli variety Takii 12 whose typical sample is a seed of a broccoli plant deposited under Accession No. FERM BP-22393, wherein the broccoli plant or the part thereof has the following characteristics (1) through (10): (1) anthocyanin coloration of leaf blade: absent; (2) anthocyanin coloration of petiole: absent; (3) intensity of anthocyanin coloration of head: weak; (4) number of leaves: many; (5) length of stem: short; (6) firmness of head: firm; (7) number of lobes: many; (8) depth of lobe: deep; (9) time of harvest: late; and (10) self-incompatibility gene: SRK¹⁸ gene and SLG¹⁸ gene.
 21. The broccoli plant or the part thereof according to claim 19, having at least 50% allele of a broccoli variety Takii 12 whose typical sample is a seed of a broccoli plant deposited under Accession No. FERM BP-22393, wherein the broccoli plant or the part thereof has at least one SNP selected from the group consisting of SNP7, SNP8, SNP20, SNP21, SNP75, SNP78, SNP81, SNP84, SNP90, SNP142, SNP165, and SNP177.
 22. The broccoli plant or the part thereof according to claim 19, having essentially all physiological and morphological characteristics of a broccoli variety Takii 12 whose typical sample is a seed of a broccoli plant deposited under Accession No. FERM BP-22393.
 23. The broccoli plant or the part thereof according to claim 20, wherein one or more characteristics have been modified.
 24. The broccoli plant or the part thereof according to claim 23, wherein the modification is performed by mutagenesis.
 25. A method for producing a seed of a broccoli plant derived from the broccoli plant according to claim 9, comprising the steps of: (a) crossing a broccoli variety Takii 12, which is a seed of a broccoli plant deposited under Accession No. FERM BP-22393, with another broccoli plant to produce a seed; (b) growing a broccoli plant from the seed obtained in step (a) to produce a broccoli plant derived from the broccoli variety Takii 12; (c) self-crossing the broccoli plant obtained in step (b) or crossing the broccoli plant obtained in step (b) with another broccoli plant to produce an additional broccoli plant derived from the broccoli variety Takii 12; and (d) optionally repeating steps (b) and (c) one or more times to further produce a broccoli plant(s) derived from the broccoli variety Takii 12, wherein the broccoli plant in step (b) has been grown from the additional broccoli plant obtained in step (c).
 26. A seed produced by the method according to claim 25, having at least 50% allele of a broccoli plant of a broccoli variety Takii 12, in which a typical sample is a seed of a broccoli plant deposited under Accession No. FERM BP-22393, wherein the broccoli plant grown from the seed has the following characteristics (1) through (10): (1) anthocyanin coloration of leaf blade: absent; (2) anthocyanin coloration of petiole: absent; (3) intensity of anthocyanin coloration of head: weak; (4) number of leaves: many; (5) length of stem: short; (6) firmness of head: firm; (7) number of lobes: many; (8) depth of lobe: deep; (9) time of harvest: late; and (10) self-incompatibility gene: SRK¹⁸ gene and SLG¹⁸ gene.
 27. A broccoli plant produced by growing the seed of the broccoli plant according to claim
 26. 28. A seed produced by the method according to claim 25, having at least 50% allele of a broccoli plant of a broccoli variety Takii 12, in which a typical sample is a seed of a broccoli plant deposited under Accession No. FERM BP-22393, wherein the broccoli plant grown from the seed has at least one SNP selected from the group consisting of SNP7, SNP8, SNP20, SNP21, SNP75, SNP78, SNP81, SNP84, SNP90, SNP142, SNP165, and SNP177.
 29. A broccoli plant produced by growing the seed of the broccoli plant according to claim
 28. 30. A method for introducing at least one new characteristic into the broccoli plant according to claim 9, comprising the steps of: (a) crossing a broccoli variety Takii 12, which is a seed of a broccoli plant deposited under Accession No. FERM BP-22393, with a broccoli plant having at least one new characteristic to produce a progeny(s); (b) selecting a progeny having at least one new characteristic; (c) crossing the progeny with the broccoli variety Takii 12 to produce a backcross progeny(s); (d) selecting a backcross progeny having at least one new characteristic and having essentially all physiological and morphological characteristics of the broccoli variety Takii 12; and (e) optionally repeating steps (c) and (d) one or more times to produce a broccoli plant(s) having at least one new characteristic and having essentially all physiological and morphological characteristics of the broccoli variety Takii 12, wherein the broccoli plant in step (c) is a backcross progeny that has been selected in step (d).
 31. A broccoli plant produced by the method according to claim
 30. 32. A method for producing a broccoli plant derived from a broccoli variety Takii 12 having at least one new characteristic, comprising the step of: transferring a mutation or a transgene that imparts at least one characteristic into a broccoli variety Takii 12, which is a seed of a broccoli plant deposited under Accession No. FERM BP-22393.
 33. A broccoli plant produced by the method according to claim
 32. 34. A method for producing a flower head of a broccoli as a food, comprising the step of: harvesting a flower head or the flower head and a peduncle of the broccoli plant according to claim
 9. 35. A processed product of the broccoli plant according to claim 9, comprising: a cut, sliced, ground, pureed, dried, canned, bottled, washed, packaged, frozen and/or heat-treated flower head.
 36. A method for determining a genotype of the broccoli plant according to claim 9 or a progeny line thereof, comprising the steps of: (a) obtaining a nucleic acid sample from the broccoli plant according to claim 9 or a progeny line thereof, and (b) detecting a polymorphism in the nucleic acid sample.
 37. A tissue culture of regenerable cells or protoplasts derived from the broccoli plant according to claim
 9. 38. The culture tissue according to claim 37, wherein the cells or protoplasts are derived from leaves, pollens, embryos, cotyledons, hypocotyls, meristematic cells, roots, root tips, anthers, flowers, seeds, or stems.
 39. A broccoli plant regenerated from the tissue culture according to claim
 38. 40. The broccoli plant according to claim 39, having essentially all physiological and morphological characteristics of a broccoli variety Takii 12, which is a seed of a broccoli plant deposited under Accession No. FERM BP-22393.
 41. A method for vegetative propagation of the broccoli plant according to claim 9, comprising the steps of: (a) collecting a propagatable tissue from a broccoli plant of a broccoli variety Takii 12, which is a seed of a broccoli plant deposited under Accession No. FERM BP-22393; (b) culturing the tissue to obtain a grown shoot; (c) rooting the grown shoot to obtain a rooted plantlet; and (d) optionally growing a plant from the rooted plantlet.
 42. A broccoli plantlet or a plant produced by the method according to claim 41, having essentially all physiological and morphological characteristics of a broccoli variety Takii 12, which is a seed of a broccoli plant deposited under Accession No. FERM BP-22393. 